S7901

Stem Cell Factor human

≥95% (SDS-PAGE), recombinant, expressed in E. coli, powder, cell culture | mammalian: suitable

• Synonym(s): SCF, c-Kit ligand
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00165757
• Biological source: human
• Recombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE)
• Form: powder
• Mol wt: monomer 18.6 kDa ((165 aa))
• Impurities: ≤1 EU/μgtested (LAL test)

Properties

• Product Name: Stem Cell Factor human, SCF, recombinant, expressed in E. coli, powder, suitable for cell culture
• biological source: human
• recombinant: expressed in E. coli
• form: powder
• quality: endotoxin tested
• packaging: pkg of 10 μg, pkg of 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: P21583
• storage temp.: −20°C
• Quality Level: 200
• assay: ≥95% (SDS-PAGE)
• potency: ≤15 ng/mL ED₅₀
• mol wt: monomer 18.6 kDa ((165 aa))
• impurities: ≤1 EU/μgtested (LAL test)

Description

Application

Stem Cell Factor human has been used in

• In vitro differentiation of equine embryonic stem cells[1]
• Chemotaxis detection system[2]
• Ex vivo expansion of umbilical cord blood CD34⁺ cells
• Cytokine therapy

Biochem/physiol Actions

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells.

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.

Epidermal stem cell factor (SCF) is considered as an essential mast cell growth factor and a multifunctional cytokine.[3] SCF mediates tumor development and progression. Upregulation of the SCF gene is observed in hepatocellular carcinoma.[3]

Physical form

Lyophilized from a 0.2 μm filtered solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5

Analysis Note

The proliferative activity is tested in culture using TF1 human erythroleukemic cells.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)