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S7901

Sigma-Aldrich

Stem Cell Factor human

SCF, recombinant, expressed in E. coli, powder, suitable for cell culture

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Synonym(s):
SCF, c-Kit ligand
MDL number:
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

>97% (SDS-PAGE)

form

powder

potency

1-5 ng/mL ED50

quality

endotoxin tested

mol wt

protein 18.5 kDa

packaging

pkg of 10 μg
pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<1 EU/μgtested (LAL test)

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... KITLG(4254)

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H8416SRP3151T7799
Sigma-Aldrich

Sigma-Aldrich

S7901

Stem Cell Factor human

Stem Cell Factor human SCF, recombinant, expressed in HEK 293 cells, suitable for cell culture

Sigma-Aldrich

H8416

Stem Cell Factor human

Sigma-Aldrich

Sigma-Aldrich

SRP3151

SCF human

recombinant

expressed in E. coli

recombinant

expressed in HEK 293 cells

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

>97% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

>95% (SDS-PAGE)

form

powder

form

lyophilized powder

form

lyophilized

form

powder

potency

1-5 ng/mL ED50

potency

≤200 ng/mL ED50

potency

≤2.0 ng/mL ED50

potency

4-20 pg/mL Activity

mol wt

protein 18.5 kDa

mol wt

dimer 35-45 kDa (glycosylated)

mol wt

18.4 kDa

mol wt

protein 18.8 kDa

Application

Stem Cell Factor human has been used in
  • In vitro differentiation of equine embryonic stem cells
  • Chemotaxis detection system
  • Ex vivo expansion of umbilical cord blood CD34+ cells
  • Cytokine therapy

Biochem/physiol Actions

Epidermal stem cell factor (SCF) is considered as an essential mast cell growth factor and a multifunctional cytokine. SCF mediates tumor development and progression. Upregulation of the SCF gene is observed in hepatocellular carcinoma.
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells.
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.

Physical form

Lyophilized from a 0.2 μm filtered solution in PBS containing 50 μg BSA per 1 μg as a carrier protein.

Analysis Note

The proliferative activity is tested in culture using TF1 human erythroleukemic cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Roberto A Rovasio et al.
European journal of cell biology, 91(5), 375-390 (2012-03-03)
The extracellular environment through which neural crest cells (NCCs) translocate and differentiate plays a crucial role in the determination of cell migration and homing. In the trunk, NCC-derived melanocyte precursor cells (MPCs) take the dorsolateral pathway and colonize the skin
Isolation and ex vivo expansion of human umbilical cord blood-derived CD34+ stem cells and their cotransplantation with or without mesenchymal stem cells.
Delalat, B, et al.
Hematology, 14.3, 375-390 (2009)
Derivation, maintenance, and induction of the differentiation in vitro of equine embryonic stem cells
Saito S, et al.
Methods in Molecular Biology, 329, 59-79 (2006)
HIF-2-dependent expression of stem cell factor promotes metastasis in hepatocellular carcinoma
Wang X, et al.
Cancer Letters, 393, 113-124 (2017)
Insights into Stem Cell Factor chemotactic guidance of neural crest cells revealed by a real-time directionality-based assay
Rovasio RA, et al.
European Journal of Cell Biology, 91, 375-390 (2012)

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