S7901

Sigma-Aldrich

Stem Cell Factor human

SCF, recombinant, expressed in E. coli, powder, suitable for cell culture

Synonym(s):
SCF, c-Kit ligand
MDL number:
NACRES:
NA.77

Quality Level

biological source

human

recombinant

expressed in E. coli

assay

>97% (SDS-PAGE)

form

powder

potency

1-5 ng/mL ED50

quality

endotoxin tested

mol wt

protein 18.5 kDa

packaging

pkg of 10 μg
pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

application(s)

cell culture | mammalian: suitable

impurities

<1 EU/μgtested (LAL test)

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... KITLG(4254)

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Application

Stem Cell Factor human has been used in
  • In vitro differentiation of equine embryonic stem cells
  • Chemotaxis detection system
  • Ex vivo expansion of umbilical cord blood CD34+ cells
  • Cytokine therapy

Biochem/physiol Actions

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells.
Epidermal stem cell factor (SCF) is considered as an essential mast cell growth factor and a multifunctional cytokine. SCF mediates tumor development and progression. Upregulation of the SCF gene is observed in hepatocellular carcinoma.

Physical form

Lyophilized from a 0.2 μm filtered solution in PBS containing 50 μg BSA per 1 μg as a carrier protein.

Analysis Note

The proliferative activity is tested in culture using TF1 human erythroleukemic cells.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Insights into Stem Cell Factor chemotactic guidance of neural crest cells revealed by a real-time directionality-based assay
Rovasio RA, et al.
European Journal of Cell Biology, 91, 375-390 (2012)
Isolation and ex vivo expansion of human umbilical cord blood-derived CD34+ stem cells and their cotransplantation with or without mesenchymal stem cells.
Delalat, B, et al.
Hematology, 14.3, 375-390 (2009)
Combining pharmacological mobilization with intramyocardial delivery of bone marrow cells over-expressing VEGF is more effective for cardiac repair
Wang Y, et al.
Journal of Molecular and Cellular Cardiology, 40, 736-745 (2006)
Th17 cell-mediated immune responses promote mast cell proliferation by triggering stem cell factor in keratinocytes.
Cho KA, et al.
Biochemical and Biophysical Research Communications, 487(4), 856-861 (2017)
Derivation, maintenance, and induction of the differentiation in vitro of equine embryonic stem cells
Saito S, et al.
Methods in Molecular Biology, 329, 59-79 (2006)
Articles
Read article on hematopoietic cytokines and hematopoiesis
Read More
Protocols
Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
Read More

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