MilliporeSigma
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S8070

Sigma-Aldrich

Anti-SUMO-1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-UBL1, Anti-Sentrin-1, Anti-SMT3H3, Anti-GMP1, Anti-SMT3C, Anti-Small Ubiquitin-related Modifiier-1, Anti-PIC1
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 14 kDa (unconjugated SUMO-1)
antigen 41 kDa (SUMO-1-GST)

species reactivity

human

technique(s)

immunoprecipitation (IP): 10-20 μg using a nuclear extract of HeLa cells
indirect immunofluorescence: 4-8 μg/mL using human HeLa cells
microarray: suitable
western blot: 0.5-1 μg/mL using nuclear extract of HeLa cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

cow ... SUMO1(614967)
human ... SUMO1(7341)
mouse ... Sumo1(22218)
rat ... Sumo1(301442)

General description

The SUMO-1 (small ubiquitin-like modifier 1) gene is mapped to human chromosome 2q33.1 and is highly conserved. The protein structure includes an α-helix core surrounded by four-stranded β-sheets.

Immunogen

synthetic peptide corresponding to amino acid 1-16 located at the N-terminus of human SUMO-1, conjugated to KLH. The sequence is identical in many species including mouse, rat, dog, bovine, and highly conserved (single amino acid substitution) in chicken and Xenopus SUMO-1. It is not found in human SUMO-2 and SUMO-3.

Application

Anti-SUMO-1 antibody produced in rabbit has been used in immunoblotting assay.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

SUMO (small ubiquitin-like modifier) proteins are associated with a number of cellular processes. SUMOylation mediated by SUMO1 is involved in nuclear transport mechanism, apoptosis, regulation of cyclin-dependent kinase 6 and protein stability. SUMO1 regulates tumor suppressor p53 protein, a transcription factor that is termed as the “guardian of the genome” plays a key role in the cell cycle events. Unlike ubiquitination which directs proteins for degradation, SUMOylation aims to alter the stability, localization and the function of the target protein.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Quotes and Ordering

Small ubiquitin-related modifier 1 is involved in hepatocellular carcinoma progression via mediating p65 nuclear translocation.
Liu J, et al.
Oncotarget, 7(16), 22206-22206 (2016)
A Central Cysteine Residue Is Essential for the Thermal Stability and Function of SUMO-1 Protein and SUMO-1 Peptide?Protein Conjugates.
Drobecq H, et al.
Bioconjugate Chemistry, 27(6), 1540-1546 (2016)
Salmonella engages host microRNAs to modulate SUMOylation: a new arsenal for intracellular survival.
Verma S, et al.
Molecular and Cellular Biology (2015)
Liberty François-Moutal et al.
Pain, 159(10), 2115-2127 (2018-05-31)
We previously reported that destruction of the small ubiquitin-like modifier (SUMO) modification site in the axonal collapsin response mediator protein 2 (CRMP2) was sufficient to selectively decrease trafficking of the voltage-gated sodium channel NaV1.7 and reverse neuropathic pain. Here, we
SUMOylation represses the transcriptional activity of the Unfolded Protein Response transducer ATF6.
Hou X, et al.
Biochemical and Biophysical Research Communications, 494(3), 446-451 (2017)

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