Documents

S8191

Sigma-Aldrich

Anti-Survivin antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):
Anti-BIRC5, Anti-TIAP
MDL number:
NACRES:
NA.46

biological source

rabbit

Quality Level

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 16 kDa

species reactivity

rat, mouse, human

application(s)

microarray: suitable
western blot: 1:500 using whole cell extract of human epitheloid carcinoma HeLa cell treated with thymidine.

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... BIRC5(332)
mouse ... Birc5(11799)
rat ... Birc5(64041)

General description

Survivin (TIAP, BIRC5), (16 kDa) is a member of the inhibitors of apoptosis (IAP)/BIR-domain-containing proteins (BIRP) gene family. It contains only one baculoviral IAP repeat domain (BIR) and lacks the domain, really interesting new gene (RING) finger. Survivin gene is localized on human chromosome 17q25.3. Survivin is highly expressed in embryonic and fetal tissues. It is selectively expressed during mitosis in the G2/M phase and is localized to mitotic spindle microtubules.

Specificity

Anti-Survivin produced using a peptide corresponding to amino acids 122-142 at the carboxyl-terminus of human survivin. This sequence is highly conserved (>70%) in rat and mouse survivin. Anti-Survivin recognizes human, mouse and rat survivin. The antibody is suitable for immunoblotting.

Immunogen

synthetic peptide corresponding to the C-terminus of human survivin (amino acids 122-142). This sequence is highly conserved (>70%) in rat and mouse survivin.

Application

Anti-Survivin antibody produced in rabbit has been used in:
  • immunohistochemistry
  • western blotting
  • immunocytochemistry

Biochem/physiol Actions

Survivin is required for cell viability maintenance in mitosis, potentially coupling apoptosis to control cell division. Antisense targeting of survivin results in increased caspase-3 activity. This occurs during mitosis, dysregulation of the centrosome, and mitotic progression, and cell death at the G2/M phase of the cell cycle. Survivin associates with cyclin-dependent kinase p34cdc2 on the mitotic apparatus, and is phosphorylated at Thr34 by p34cdc2-cyclin B1. . Survivin is overexpressed in a variety of human tumors, including adenocarcinomas of lungs, pancreas, breast, colorectal and stomach. It is also present in elevated levels in prostate, high-grade non-Hodgkin lymphoma, and neuroblastomas. Expression of a phosphorylation defective survivin mutant Thr34 to Ala triggers apoptosis in human melanoma cell lines in vitro and in vivo and inhibits melanoma tumor growth in mice. This makes survivin to be useful in cancer therapy by its direct effect anti-apoptotic pathway.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

2

Certificate of Analysis

Certificate of Origin

Demographic characteristics, risk factors and immunocytochemistry of p 16INK4a, Ki-67, MCM5, and survivin as predictors for the progress of cervical precancer lesion
Indarti J, et al.
Medical Journal of Indonesia, 19(3) (2010)
D Grossman et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(2), 635-640 (2001-01-10)
A role of apoptosis (programmed cell death) in tumor formation and growth was investigated by targeting the apoptosis inhibitor survivin in vivo. Expression of a phosphorylation-defective survivin mutant (Thr(34)-->Ala) triggered apoptosis in several human melanoma cell lines and enhanced cell...
Hai-Ning Zhen et al.
Cancer, 104(12), 2775-2783 (2005-11-15)
An unbalance of cell proliferation and cell apoptosis is an important mechanism in carcinogenesis, and angiogenesis also plays a crucial role in tumorigenesis. Recently, survivin has been identified as an important member of the inhibitor of apoptosis protein (IAP) family....
Roberta Soares Faccion et al.
Cancer investigation, 30(5), 404-414 (2012-05-11)
Centroblastic diffuse large B cell lymphoma (DLBCL) samples were analyzed by immunohistochemistry to evaluate the expression of p53, Bcl-2, Survivin, XIAP, and Ki-67. Survivin was the only protein which expression exhibited a trend for impact in progression-free (p = .077)...
Nathan R Wall et al.
Cancer research, 63(1), 230-235 (2003-01-09)
Survivin is a member of the inhibitor of apoptosis gene family that is expressed in most human cancers and may facilitate evasion from apoptosis and aberrant mitotic progression. Here, exposure of breast carcinoma MCF-7 or cervical carcinoma HeLa cells to...

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