MilliporeSigma
All Photos(1)

S8321

Sigma-Aldrich

Anti-Sonic Hedgehog antibody, Mouse monoclonal

clone SH154, purified from hybridoma cell culture

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
Anti-Shh
MDL number:

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

SH154, monoclonal

form

buffered aqueous solution

species reactivity

human, rat, mouse

concentration

~2 mg/mL

technique(s)

immunohistochemistry: 1:500 using formaldehyde-fixed human tissue sections
indirect ELISA: 2 μg/mL
western blot: 2 μg/mL using recombinant mouse SHH peptide

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SHH(6469)
mouse ... Shh(20423)
rat ... Shh(29499)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SAB1300534S9822SAB5701044
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

antibody form

purified immunoglobulin

antibody form

saturated ammonium sulfate (SAS) precipitated

antibody form

affinity isolated antibody

antibody form

-

clone

SH154, monoclonal

clone

polyclonal

clone

polyclonal

clone

monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

lyophilized powder

form

liquid

species reactivity

human, rat, mouse

species reactivity

human

species reactivity

mouse

species reactivity

human, mouse, rat

General description

The Hedgehog (Hh) family of secreted proteins consists of sonic hedgehog (Shh), desert hedgehog (Dhh) and Indian hedgehog (Ihh). The Hh signaling regulates cell migration and tissue construction during embryogenesis and reconstruction in adults. Shh protein is secreted by Purkinje cells in the cerebellum with both autocrine and paracrine functions. The Shh signaling is mediated by the interaction of Shh with transmembrane proteins, Patched and Smoothened. The Shh pathway cross-talks Notch and Wnt pathways and acts via downstream targets such as cyclins, γ catenin, myc and other transcription factors. The Hh family members have multiple roles as morphogens, cell survival factors, setting anterior-posterior axis and digit formation during development of limb and axon guidance factors during the development of brain and nervous system. Hedgehog signaling has been implicated in childhood tumors of pancreas, liver, thyroid, gastrointestinal tract, neuroblastomas and medulloblastomas
Anti-Sonic Hedgehog antibody recognizes human, rat and mouse Shh.

Specificity

Mouse monoclonal clone SH154 anti-Sonic Hedgehog antibody recognizes human, rat, and mouse Shh.

Application

Anti-Sonic Hedgehog antibody may be used for immunoblotting at a working concentration of ~2 μg/ml. A working antibody concentration of ~2 μg/ml is recommended for ELISA. For immunohistochemistry a dilution of 1:500 may be used.
Mouse monoclonal clone SH154 anti-Sonic Hedgehog antibody may be used in ELISA, immunoblotting, and immunohistochemistry. It is used as a probe to determine the presence and roles of Sonic Hedgehog in processes such as patterning during embryogenesis.

Biochem/physiol Actions

Sonic Hedgehog (Shh) is an important cell signaling molecule expressed during embryonic development. Shh is involved in patterning of the developing embryonic systems such as the nervous system, somites, and limbs. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened. Shh protein is expressed in key embryonic tissues such as the Hensen′s node, zone of polarizing activity in the posterior limb bud, notochord, and floor plate of the neural tube. Downstream targets of Shh include the transcription factors Gli3, responsible for Greigs polycephalosyndactyly in humans, and Hoxd13, responsible for polysyndactyly.

Physical form

Solution in 0.01 M phosphte buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Abu Shadat M Noman et al.
Therapeutic advances in medical oncology, 12, 1758835920911229-1758835920911229 (2020-03-25)
Sonic hedgehog (Shh) and Nrf2 play a critical role in chemotherapeutic resistance. These two genes have been found to be dysregulated in head and neck squamous cell carcinomas (HNSCC). The purpose of this study was to analyze the expression, function
Role of Sonic hedgehog signaling pathway in neuroblastoma development
Shahi MH et al
Biol. Med., 1, Rev2-Rev2 (2009)
Shih-Hung Yang et al.
Oncology, 85(2), 86-94 (2013-07-19)
We investigated the association of the hedgehog pathway with nuclear factor (NF)-κB and clinical outcomes in pancreatic cancer patients. We analyzed tissue samples for the expression of NF-κB (RelA/p65), sonic hedgehog (Shh) and glioma-associated oncogene homolog 1 (Gli1) by immunohistochemistry
Xinyue Hu et al.
Gut microbes, 15(1), 2221093-2221093 (2023-06-07)
Epithelial ovarian cancer (EOC) is the most lethal gynecological cancer, which remains a threat to female health at all ages. Hypotheses for EOC development include the continuous presence of inflammation, in which microbiota and inflammatory cytokines participate in cancer-related signaling
Abu Shadat M Noman et al.
Therapeutic advances in medical oncology, 12, 1758835920911229-1758835920911229 (2020-03-25)
Sonic hedgehog (Shh) and Nrf2 play a critical role in chemotherapeutic resistance. These two genes have been found to be dysregulated in head and neck squamous cell carcinomas (HNSCC). The purpose of this study was to analyze the expression, function

Articles

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service