S8406

Sigma-Aldrich

Stromal Cell-Derived Factor 1β/pre-B Cell Growth Stimulating Factor human

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):
SDF-1β/PBSF
MDL number:
NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

biological source

human

recombinant

expressed in E. coli

assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

10-30 ng/mL ED50

mol wt

~8 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

application(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... CXCL12(6387)

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General description

C-X-C motif chemokine ligand 12 (CXCL12), also known as stromal cell-derived factor 1 α (SDF-1a), is encoded by the gene mapped to human chromosome 10q11. Stromal cell-derived factor 1 α and β (SDF-1 α and β) are stromal derived CXC chemokines and signal through the C-X-C motif chemokine receptor-4 (CXCR4). CXCL12 is mainly expressed in the inflamed and failing myocardium.

Application

Stromal Cell-Derived Factor 1β has been used to activate chemokine receptor-4 (CXCR4) and to further assess its role in tumor cell migration using chemotaxis assay.

Biochem/physiol Actions

SDF-1/PBSF is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. It is highly conserved between species, with only one amino acid substitution between the human and mouse proteins.
C-X-C motif chemokine ligand 12 (CXCL12) is involved in inflammation, proliferation, tumorigenicity and metastasis. CXCL12 exerts HIV (human immunodeficiency virus) suppressive activity in cells expressing the CXCR4 (C-X-C motif chemokine receptor 4). It is overexpressed in breast carcinoma, ovarian carcinoma and papillary thyroid carcinoma. It enhances angiogenesis in the ischemic tissue. SDF1 is also implicated in neuroprotection after ischemic stroke with the help of CXCR4.
SDF-1/PBSF, a member of the α (CXC) chemokine family, is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1α and SDF-1β (together also known as SDF-1/PBSF) are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the C-terminal of SDF-1β and absent from SDF-1α. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the human and mouse proteins.

Physical form

Lyophilized from 30% acetonitrile and 0.1% trifluoracetic acid containing 0.5 mg of bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to chemoattract 8-10 day human T lymphocytes cultured in the presence of IL-2.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Gene-environment interaction effects on the development of immune responses in the 1st year of life.
American Journal of Human Genetics (2005)
Stromal cell-derived factor 1a (SDF-1a): A marker of disease burden in patients with atrial fibrillation.
Li D
Scandinavian Cardiovascular Journal : SCJ (2016)
The diagnostic and prognostic value of serum CXCL12 levels in patients with ischemic stroke.
Duan XX
Neurological Sciences, 36(12) (2015)
Dermal fibroblast expression of stromal cell-derived factor-1 (SDF-1) promotes epidermal keratinocyte proliferation in normal and diseased skin.
Quan C
Protein & cell (2015)
Wnt/?-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells.
International Journal of Clinical and Experimental Pathology (2015)

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