S8571

Sigma-Aldrich

Anti-Seladin-1 antibody produced in rabbit

enhanced validation

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):
Anti-24-dehydro-cholesterol reductase (DCHR24), Diminuto/Dwarf-1 homolog, Anti-Selective Alzheimer’s Disease Indicator-1
MDL number:
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

rabbit

Quality Level

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~60 kDa

species reactivity

human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

application(s)

western blot: 1:4,000-1:8,000 using HEK293 cells expressing human seladin-1.

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... DHCR24(1718)

General description

Seladin-1 (selective Alzheimer′s disease indicator-1) is an anti-apoptotic gene. It is a human homolog of the Diminuto/Dwarf1 gene described in plants andC. elegans. Seladin-1 is encoded by the DHCR24 (3-b-hydroxysterol-D-24-reductase) gene, a flavin-adenine-dinucleotide (FAD)-dependent oxidoreductase. It is mainly located in the endoplasmic reticulum.

Immunogen

synthetic peptide corresponding to amino acids 68-85 of human seladin-1, conjugated to KLH.

Application

Anti-Seladin-1 antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Seladin-1 (selective Alzheimer′s disease indicator-1) catalyzes the conversion of desmosterol to cholesterol. It prevents apoptosis via inhibition of caspase-3, a key mediator of the apoptotic cascade, suggesting that seladin-1 may be involved in the regulation of cell survival and death. Decreased expression of seladin-1 in specific neurons is linked to Alzheimer′s disease (AD). Seladin-1 functions as a key mediator of Ras-induced senescence in mouse and human fibroblasts. It protects neurons from β-amyloid toxicity and oxidative stress. Seladin-1 is involved in cholesterol homeostasis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
The human homolog of Diminuto/Dwarf1 gene (hDiminuto): a novel ACTH-responsive gene overexpressed in benign cortisol-producing adrenocortical adenomas
Sarkar D, et al.
The Journal of clinical endocrinology and metabolism, 86(11), 5130-5137 (2001)
The role of seladin-1/DHCR24 in cholesterol biosynthesis, APP processing and Abeta generation in vivo
Crameri A, et al.
The Embo Journal, 25(2), 432-443 (2006)
I Greeve et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 20(19), 7345-7352 (2000-09-29)
In Alzheimer's disease (AD) brains, selected populations of neurons degenerate heavily, whereas others are frequently spared from degeneration. To address the cellular basis for this selective vulnerability of neurons in distinct brain regions, we compared gene expression between the severely...
Articles
Alzheimer's disease (AD) is the most common cause of dementia in the elderly and is characterized by gradual loss of cognitive functions.
Read More

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