Selective Alzheimer′s disease indicator-1 (seladin-1) protein is encoded by the 3-β-hydroxysterol-24-reductase (DHCR24) gene. This protein is mainly located in the endoplasmic reticulum. Seladin-1 contains a highly conserved flavin adenine dinucleotide (FAD)-binding domain. DHCR24 gene is located on human chromosome 1p32.3. This gene contains 8 introns and 9 exons.
Anti-Seladin-1(C-terminal) recognizes human seladin-1.
synthetic peptide corresponding to amino acids 505-516 located in a region near the C-terminus of human seladin-1.
Anti-Seladin-1 (C-terminal) antibody produced in rabbit may be used in immunoblotting.
Selective Alzheimer′s disease indicator-1 (seladin-1) protein catalyzes the conversion of desmosterol to cholesterol. It can protect neurons from β-amyloid toxicity and oxidative stress. In addition, it prevents apoptosis via inhibition of caspase-3, stating that seladin-1 may be involved in the regulation of cell survival and death. Seladin-1 aids to mediate Ras-induced senescence in mouse and human fibroblasts. This protein is upregulated in adrenocortical adenomas and in some tumors. DHCR24 participates plays a key role in cell differentiation, antiapoptotic function, and anti-inflammatory activity. Seladin-1 is known to involve in cholesterol biosynthesis l. A defect in DHCR24 enzyme results in desmosterolosis.
Seladin-1 (C-terminal) is an anti-apoptotic gene, found to be down regulated in large pyramidal neurons in brain regions affected by AD, and to be involved in the regulation of cellular response tooncogenic and oxidative stress.
Solution in 0.01 M phosphate buffered saline, pH 7.4, and containing 15 mM sodium azide.
Storage and Stability
For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. For continuous use, store at 2-8 °C for up to one month.
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