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S8761

Sigma-Aldrich

Sodium bicarbonate solution

solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture

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Synonym(s):
aqueous sodium bicarbonate
CAS Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

sterility

sterile-filtered

product line

BioReagent

form

solution (7.5%)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

7.8-8.2

storage temp.

2-8°C

SMILES string

[Na+].OC([O-])=O

InChI

1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1

InChI key

UIIMBOGNXHQVGW-UHFFFAOYSA-M

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This Item
S5761364861.06323
Sodium bicarbonate powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture

Sigma-Aldrich

S5761

Sodium bicarbonate

Sodium hydrogen carbonate EMPROVE® ESSENTIAL, Ph. Eur., BP, ChP, JP, USP, FCC, E 500

SAFC

1.06323

Sodium hydrogen carbonate

product line

BioReagent

product line

BioReagent

product line

-

product line

EMPROVE® ESSENTIAL

form

solution (7.5%)

form

powder

form

liquid

form

solid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

ion chromatography: suitable

technique(s)

-

impurities

endotoxin, tested

impurities

-

impurities

-

impurities

-

pH

7.8-8.2

pH

6.8-7.4

pH

-

pH

8.6 (20 °C, 50 g/L in H2O)

General description

Sodium bicarbonate (NaHCo3) is a commonly used alkaline buffering agent(5) in laboratories. The addition of this buffer to the cell culture medium aids in maintaining the appropriate pH by using the carbon dioxide present in the incubator. In addition to its buffering properties, Sodium bicarbonate also acts as a source of sodium and aids in sodium gluconate synthesis during the fermentation process.

Application

Sodium bicarbonate solution has been used:
  • as a component of Hanks′ buffered salt solution (HBSS) and recording medium for culturing of tissue explants from rats or mice
  • as a component of Dulbecco′s modified Eagle′s medium (DMEM) to culture mice tissue explants
  • as a component of Minimum Essential Media (MEM) to study the cytotoxicity against human colorectal adenocarcinoma cells and mammary gland adenocarcinoma
  • to prepare collagen mix droplets for 3D collagen sprouting assay
  • to prepare collagen matrix for rat epidermal keratinocyte (REK) organotypic culture

Preparation Note

Prepared in cell culture grade water.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Protocols

3-D Culture Matrix™ Collagen I may be used as a gel on which to grow cells or a media additive alone or in concert with other basement membrane components to study cellular growth and differentiation in three dimensions in vitro.

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