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S8820

Sigma-Aldrich

SIGMAFAST™ Protease Inhibitor Tablets

For General Use

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Synonym(s):
protease inhibitor
NACRES:
NA.77

biological source

bovine lung
bovine milk

Quality Level

200

form

tablet

solubility

water: 1 tablet/100mL, clear to hazy, colorless

storage temp.

2-8°C

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SIGMAFAST™ Protease Inhibitor Tablets For General Use

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SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free for use in purification of Histidine-tagged proteins

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SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free

ReadyShield® Protease Inhibitor Cocktail for use in tissue culture media, Non-freezing solution

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form

tablet

form

tablet

form

ready-to-use solution

form

(non-freezing liquid formulation )

solubility

water: 1 tablet/100mL, clear to hazy, colorless

solubility

-

solubility

-

solubility

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

-

Quality Level

-

General description

SIGMAFAST™ Protease Inhibitor Tablets are a water-soluble tablet formulation designed to inhibit serine, cysteine, aspartic, and metalloproteases. The tablets contain a broad-spectrum cocktail of protease inhibitors, including AEBSF, E-64, bestatin, leupeptin, aprotinin, and EDTA. One tablet can make 100 mL of cocktail, and can inhibit proteases present in up to 20 g of cell extract.

Specificity

Inhibits serine, cysteine, aspartic, and metalloproteases.

Application

These tablets have been optimized and tested for general use. They have been used in various applications, including collecting tracheae in combination with ice-cold radioimmunoprecipitation assay buffer to determine the tracheal basal cell fate, and homogenizing rat lung tissues for western blot analysis.

Features and Benefits

Broad-spectrum protease inhibition: Inhibits serine, cysteine, aspartic, and metalloproteases, making it a versatile solution for protease inhibition in various applications.

Water-soluble tablet formulation: Easy to use and prepare, with one tablet making 100 mL of cocktail.

Contains a cocktail of protease inhibitors: Provides a comprehensive and effective solution for protease inhibition.

Tested and optimized for general use: Proven performance and reliability for a wide range of applications.

Components

AEBSF, 2 mM
Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM

Other Notes

Tableted formulation containing water-soluble protease inhibitors with broad specificity for the inhibition of serine, cysteine, and metalloproteases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), E-64, bestatin, leupeptin, aprotinin, and EDTA (sodium salt).

Quantity

One tablet makes 100 mL of cocktail. One mL is recommended for the inhibition of proteases equivalent to 1 mg of USP pancreatin. One tablet is recommended for the inhibition of proteases present in a maximum of 20 g of cell extract.

Physical form

Tablet that is water soluble.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

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P8215

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Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H314 - H335

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1A - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Articles

Aprotinin

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

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