SIGMAFAST Protease Inhibitor Tablets

For General Use


Quality Level

biological source

bovine lung
bovine milk




water: 1 tablet/100mL, clear to hazy, colorless

storage temp.



Inhibits serine, cysteine, aspartic, and metalloproteases.


SIGMAFAST Protease Inhibitor Tablets has been used to collect tracheae in combination with ice-cold radioimmunoprecipitation assay buffer in a study to determine the tracheal basal cell fate. It has been used to homogenize rat lung tissues for western blot analysis.
Optimized and tested for general use.


Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM

Other Notes

Tableted formulation containing water-soluble protease inhibitors with broad specificity for the inhibition of serine, cysteine, and metalloproteases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), E-64, bestatin, leupeptin, aprotinin, and EDTA (sodium salt).


One tablet makes 100 mL of cocktail. One mL is recommended for the inhibition of proteases equivalent to 1 mg of USP pancreatin. One tablet is recommended for the inhibition of proteases present in a maximum of 20 g of cell extract.

Physical form

Tablet that is water soluble.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC



Signal Word


Hazard Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the difference between Protease Inhibitors S8820 and P2714?

    S8820 is the tablet version of P2714.  

  6. Can Product S8820, SIGMAFAST Protease Inhibitor Tablets, be used with with HIS-Select products or any other immobilized metal affinity chromatography (IMAC) products?

    The tablets contain EDTA, a metal chelator, which may remove metal ions from these affinity products. The suggested protease inhibitor for the HIS-Select products is either Product No. S8830 (water soluble tablet) or P8849 (DMSO solution).

  7. Can Product S8820, SIGMAFAST Protease Inhibitor Tablets, be used with ELISA?

    Yes, because it is water based.

  8. How should I dissolve Product S8820, SIGMAFAST Protease Inhibitor Tablets?

    You can dissolve the product in 10 ml of water to make a 10X stock solution and then dilute 1:10 for use.

  9. What is the concentration of the inhibitors in Product S8820, SIGMAFAST Protease Inhibitor Tablets?

    When reconstituted (one tablet in 100 ml water), concentrations of the component inhibitors are as follows:AEBSF      2 mME-64          14 μMBestatin     130 μMLeupeptin   0.9 μMAprotinin    0.3 μMEDTA        1 mM

  10. How much of Product S8820, SIGMAFAST Protease Inhibitor Tablets, needs to be used in cell lysates?

    One tablet is recommended for the inhibition of proteases present in a maximum of 20 grams of cell extract.

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Artavazd Tadevosyan et al.
The Journal of biological chemistry, 285(29), 22338-22349 (2010-05-14)
Angiotensin-II (Ang-II) from extracardiac sources and intracardiac synthesis regulates cardiac homeostasis, with mitogenic and growth-promoting effects largely due to altered gene expression. Here, we assessed the possibility that angiotensin-1 (AT1R) or angiotensin-2 (AT2R) receptors on the nuclear envelope mediate effects...
Jan Philipp Novotný et al.
PeerJ, 5, e3789-e3789 (2017-10-05)
Heather M Brechbuhl et al.
The American journal of pathology, 179(1), 367-379 (2011-06-28)
The purpose of this study was to determine whether β-catenin regulates basal cell fate determination in the mouse trachea. Analysis of TOPGal transgene reporter activity and Wnt/β-catenin pathway gene expression suggested a role for β-catenin in basal cell proliferation and...
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
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