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S8830

Sigma-Aldrich

SIGMAFAST Protease Inhibitor Cocktail Tablets, EDTA-Free

for use in purification of Histidine-tagged proteins

Synonym(s):
Protease Inhibitor Cocktail
MDL number:
NACRES:
NA.77

Quality Level

biological source

bovine lung

form

tablet

storage temp.

2-8°C

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General description

SIGMAFAST Protease Inhibitor Cocktail Tablets, EDTA-Free is a tableted formulation containing water-soluble protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic and metalloproteases. The tablets have been formulated to be compatible with sample processing by immobilized metal affinity chromatography (IMAC) such as used for the purification of histidine-tagged proteins. The tablets contain no strong metal chelators, such as EDTA, which might interfere with IMAC processing. Finally, the tablets contain a unique, proprietary formulation including a pepstatin A salt to provide additional protection not normally obtained in a tablet format.

Specificity

Inhibits serine, cysteine, aspartic, and metalloproteases.

Application

The broad protection afforded allows SIGMAFAST Protease Inhibitor Cocktail Tablets to be used for the purification of histidine-tagged proteins, as a general-purpose protease inhibitor cocktail and as components of buffers for cell lysis. The tablets have also been optimized and tested for use with HIS-Select media.

Components

AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon
Leupeptin
Aprotinin

Quantity

One tablet makes 100 mL of cocktail. One tablet is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract.

Physical form

Tablet that is water soluble

Storage and Stability

The tablets are stable as supplied for at least 2 years at 2–8 °C. The reconstituted protease inhibitor solution (1´ or 10´) is stable for at least 2 weeks at 2–8 °C.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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SDS

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