Safranin O stain for cartilage requires a specific procedure involving fixative, deparaffinization, staining, and mounting. The use of acetic acid after staining sets the stain to the tissue.
S8884
Safranin O
Dye content, ≥80%, certified by the Biological Stain Commission, powder
Synonym(s):
Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A
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About This Item
Empirical Formula (Hill Notation):
C20H19ClN4
CAS Number:
Molecular Weight:
350.84
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
207-518-8
MDL number:
Colour Index Number:
50240
Beilstein/REAXYS Number:
3924099
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Product Name
Safranin O, certified by the BSC
InChI key
OARRHUQTFTUEOS-UHFFFAOYSA-N
InChI
1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
SMILES string
[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N
agency
certified by the BSC
form
powder
composition
Dye content, ≥80%
technique(s)
microbe id | staining: suitable
color
green to very dark green
solubility
H2O: 1 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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This Item | |||
|---|---|---|---|
| grade certified by the Biological Stain Commission | grade - | grade - | grade for microscopy |
| technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) - | technique(s) microbe id | staining: suitable |
| color green to very dark green | color - | color - | color red to very dark red |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| form powder | form powder | form solid | form liquid |
| solubility H2O: 1 mg/mL | solubility water: 1 mg/mL, clear, dark red to very dark red and red purple | solubility 50 g/L | solubility water: soluble at 20 °C |
General description
Safranin O is a metachromatic, cationic dye. It is used as a counterstain in Gram staining. The stain colors Gram-negative bacteria pink to red and has no effect on Gram-positive bacteria. It is also used to assess glycosaminoglycans (GAGs) in cartilage. The dye imparts an intense red to GAGs. It easily binds to polyanions.[1][2]
Application
Certified as a stain for chromosomes in the Flemming triple stain; to stain gram negative bacteria in the Gram stain procedure; Safranine-fast green FCF stain for plant cells.
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Danger
hcodes
Hazard Classifications
Eye Dam. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Is there a staining protocol available for staining cartilage, particularly for decalcified bone (joint tissue) using CalX, and subsequent staining with Fast green and Safranin O?
1 answer-
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