S9194

SYBR^® Green JumpStart^™ Taq ReadyMix^™ for High Throughput qPCR

SYBR^® Green qPCR reagent, passive reference dye included

• NACRES: NA.55

Properties

• Quality Level: 200
• form: liquid
• usage: sufficient for 20 reactions; sufficient for 2000 reactions; sufficient for 400 reactions
• feature: dNTPs included; hotstart
• concentration: 1.25 units/reaction (50 μL reaction volume)
• technique(s): qPCR: suitable
• color: colorless
• input: purified DNA
• compatibility: for use with ABI 5700; for use with ABI 7000; for use with ABI 7300; for use with ABI 7700; for use with ABI 7900 Fast; for use with ABI 7900 HT; for use with ABI 7900; for use with ABI StepOne; for use with ABI StepOnePlus
• detection method: SYBR^® Green
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

SYBR^® Green JumpStart^™ Taq ReadyMix^™ is a ready-to-use 2X master mix that contains SYBR^® Green I, JumpStart^™ Taq DNA polymerase, 99% pure deoxynucleotides (dNTPs), and reaction buffer making it the ideal solution for performing high-throughput quantitative PCR. SYBR^® Green I dye binds to and detects any dsDNA generated during amplification. The JumpStart^™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.

Application

SYBR^® Green JumpStart^™ Taq ReadyMix^™ for High Throughput qPCR has been used for the amplification and quantification of transcripts to analyze gene expression in 2-step quantitative reverse transcription PCR (RT-qPCR). It has also been used for quantitative polymerase chain reaction (qPCR) of reverse-transcribed cDNA.

Features and Benefits

• The master mix allows consistency and reproducibility from one reaction to the next
• Reduced set-up time as compared to manual or wax Hot Start methods
• Reduced primer dimers
• Allows for room temperature set-up; Ideal for high throughput, quantitative PCR application

• SYBR^® Green I dye binds to double-stranded DNA and is ideal for quantifying any DNA sequence. Detection is monitored by measuring the increase in fluorescence throughout cycling.
• JumpStart Taq DNA polymerase prevents amplification of non-specific products while increasing target yield.
• Internal Reference Dye is provided for reaction normalization. Maximum excitation and emission is 586 nm and 605 nm, respectively.
• SYBR Green JumpStart Taq ReadyMix reduces preparation time and the risk of contamination from multiple pipetting steps.

Packaging

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
400RXN is packaged as 1 X 10 mL
2000RXN is packaged as 1 X 50 mL

Other Notes

SYBR Green ReadyMix for High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq and SYBR Green I in an easy-to-use ReadyMix solution that incorporates ROX dye for ABI and other real time instrument applications. The ReadyMix includes a detection fluor, internal standard and reagents for PCR making it the ideal solution for performing high-throughput quantitative PCR.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Life Technologies