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S9626

Sigma-Aldrich

Sulfatase from Helix pomatia

Type H-1, sulfatase ≥10,000 units/g solid

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Synonym(s):
Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

type

Type H-1

Quality Level

form

powder

sulfatase activity

≥10,000 units/g solid

secondary activity

≥300 unit/mg solid β-glucuronidase (at pH 5.0)

mol wt

~85 kDa by gel filtration

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

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S1629S9751SRE0020
vibrant-m

S9626

Sulfatase from Helix pomatia

vibrant-m

S1629

Sulfatase from Aerobacter aerogenes

vibrant-m

S9751

Sulfatase from Helix pomatia

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

400, 600

mol wt

~85 kDa by gel filtration

mol wt

~41 kDa

mol wt

~85 kDa by gel filtration

mol wt

-

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

application(s)

diagnostic assay manufacturing

Application

Sulfatase from Helix pomatia has been used in:
  • sample extraction
  • sulfatase assays
  • enzymatic hydrolysis
  • extract preparation for glucosinolate analysis

Biochem/physiol Actions

Sulfatase from Helix pomatia facilitates the synthesis of thiohydroximates. It causes the hydrolysis of β-naphthyl sulfate much faster than a-naphthyl sulfate and possesses regiospecificity in the hydrolysis of ortho and para substituted phenyl sulfates.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).

substrate

Product No.
Description
Pricing

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)


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Ivica Blažević et al.
Phytochemistry, 169, 112100-112100 (2019-11-28)
The glucosinolates (GSLs) is a well-defined group of plant metabolites characterized by having an S-β-d-glucopyrano unit anomerically connected to an O-sulfated (Z)-thiohydroximate function. After enzymatic hydrolysis, the sulfated aglucone can undergo rearrangement to an isothiocyanate, or form a nitrile or
A single monooxygenase, ese, is involved in the metabolism of the organochlorides endosulfan and endosulfate in an Arthrobacter sp.
Weir K M, et al.
Applied and Environmental Microbiology, 72(5), 3524-3530 (2006)
Stephen S Hecht et al.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 13(6), 997-1004 (2004-06-09)
Vegetable consumption, including cruciferous vegetables, is protective against lung cancer, but the mechanisms are poorly understood. The purpose of this study was to investigate the effects of cruciferous vegetable consumption on the metabolism of the tobacco-specific lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)
Simultaneous analysis of several non-steroidal anti-inflammatory drugs in human urine by high-performance liquid chromatography with normal solid-phase extraction
Hirai T, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 692(2), 375-388 (1997)
Glucosinolate enhancement in cabbage induced by jasmonic acid application.
Fritz, V. A., Justen, V. L., Bode, A. M., Schuster, T., & Wang, M.
Hortscience: a Publication of the American Society For Horticultural Science Hortscience, 45, 1188-1191 (2010)

Protocols

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

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