S9915

Sigma-Aldrich

Stem Cell Factor from mouse

SCF, recombinant, expressed in E. coli, powder, suitable for cell culture

Synonym(s):
SCF, c-Kit ligand
MDL number:
NACRES:
NA.77
Pricing and availability is not currently available.

Quality Level

biological source

mouse

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE and HPLC)

form

powder

potency

≤10 ng/mL ED50/EC50

quality

endotoxin tested

mol wt

18.3 kDa

packaging

pkg of 10 μg
pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

application(s)

cell culture | mammalian: suitable

impurities

<1 EU/μg

color

white

solubility

water: soluble

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... Kitl(17311), kitl(17311)

General description

Stem cell factor (SCF), also known as the c-kit ligand, is produced by the gastrointestinal smooth muscle cells, enteric neurons, endothelial cell, lung fibroblast and bone marrow adipocytes. The SCF gene is mapped to murine chromosome 10.
Recombinant mouse stem cell factor (SCF) is a 18.3 kDa protein containing 164 amino acid residues.

Application

SCF, also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.
Stem Cell Factor from mouse has been used in the evaluation of in vitro 5-lipoxygenase inhibition by measuring the formation of LTC4.

Biochem/physiol Actions

Stem cell factor is responsible for cellular development, proliferation, migration and survival, specially observed with cardiac stem cells. This phenomenon is mediated by stem cell factor (SCF) through chemotaxis. SCF is involved in repair mechanism of myocardial infarctions. SCF signalling is responsible for the function and survival rate of interstitial cells of Cajal. Decreased level of SCF leads to gastrointestinal motility disorders. SCF might be associated with systemic vascular remodeling. SCF signalling is also believed to contribute to the pathogenesis of pulmonary hypertension.
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells.

Physical form

Lyophilized from a 0.2 μm filtered buffered solution.

Analysis Note

The biological activity is measured by the dose-dependent stimulation of the proliferation of human TF-1 cells.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Persistent distention of colon damages interstitial cells of Cajal through Ca2+-ERK-AP-1-miR-34c-SCF deregulation
Yang S, et al.
Journal of Cellular and Molecular Medicine, 21(9), 1881-1892 (2017)
Antagonism of Stem Cell Factor/c-kit Signaling Attenuates Neonatal Chronic Hypoxia-Induced Pulmonary Vascular Remodeling
Young KC, et al.
Pediatric Research, 79(4), 637-646 (2016)
Edyta E Wojtowicz et al.
Experimental hematology, 42(10), 909-918 (2014-08-06)
MicroRNAs (miRNAs) are crucial for proper functioning of hematopoietic stem and progenitor cells (HSPCs). Members of the miRNA-125 family (consisting of miR-125a, miR-125b1, and miR-125b2) are known to confer a proliferative advantage on cells upon overexpression, to decrease the rate...
SCF/c-kit transactivates CXCR4-serine 339 phosphorylation through G protein-coupled receptor kinase 6 and regulates cardiac stem cell migration
Zuo K, et al.
Scientific Reports, 6: 26812, 1-12 (2016)
Synthesis and evaluation of benzoxazole derivatives as 5-lipoxygenase inhibitors
Song H, et al.
Bioorganic & Medicinal Chemistry, 18, 7580-7585 (2010)

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