SAB1300086

Anti-SOST (N-term) antibody produced in rabbit

Ig fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-CDD, Anti-DAND6, Anti-SOST1, Anti-VBCH
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ELISA (i), IHC, WB
• Citations: 7

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: Ig fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: mouse, human
• technique(s): immunohistochemistry: 1:50-1:100, indirect ELISA: 1:1000, western blot: 1:100-1:500
• NCBI accession no.: NP_079513
• UniProt accession no.: Q9BQB4
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Quality Level: 100
• Gene Information: human ... SOST(50964)

Description

General description

SOST (sclerostin) gene codes for the protein sclerostin which is predominantly secreted by the osteocytes. The encoded protein is predominantly expressed in human woven and lamellar bone and mineralizing chondrocytes.

Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.

Immunogen

SOST (NP_079513, 12-42)
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SOST.

Application

Anti-SOST (N-term) antibody produced in rabbit has been used in immunohistochemistry.

Biochem/physiol Actions

Sclerostin regulates bone remodeling in presence of mechanical stimulation. It has an inhibitory effect on the activation of osteoblasts by inducing phosphorylation and degradation of β-catenin. It downregulates bone formation and induces bone resorption. Mutation in this gene causes hyperostosis and leads to Van Buchem disease or sclerosteosis. Increased bone formation and enhanced healing of bone defects are observed upon selective deletion of the SOST gene in mice model. Sclerostin, a glycoprotein, is involved in vascular calcification. It might be associated with cardiovascular disorders.

Physical form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable