SAB1300355

Sigma-Aldrich

Anti-Drosophila Parkin (C-term) antibody produced in rabbit

saturated ammonium sulfate (SAS) precipitated, buffered aqueous solution

Synonym(s):
Anti-Cg10523-pc, Anti-PARKIN, Anti-CG10523-PB, Anti-Isoform B, Anti-Isoform c
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

saturated ammonium sulfate (SAS) precipitated

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

Drosophila

application(s)

indirect ELISA: 1:1000
western blot: 1:100-1:500

conjugate

unconjugated

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

General description

Parkin is thought to play a role in the ubiquitin/proteasome pathway for protein degradation. The amino terminus bears sequence homology to ubiquitin while functionally it acts as a RING-type ubiquitin protein ligase (E3) that coordinates the transfer of ubiquitin to substrate proteins, thus targeting them for degradation by the proteasome. Mutations in the human version of the protein are known to cause autosomal recessive juvenile parkinsonism.
Parkin gene is located on Drosophila melanogaster chromosome 78C2. This gene codes for the enzyme E3 ubiquitin ligase, which localizes in the cytosol.

Immunogen

PARKIN (NP_730600, )
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of Drosophila Parkin.

Application

Anti-Drosophila Parkin (C-term) antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

Mutation in the parkin gene leads to Parkinson′s disease. The encoded protein is involved in quality control mechanism for the disposal of abnormal mitochondria, thereby maintaining lifespan and controlling locomotion. Parkin along with PTEN-induced putative kinase 1 (PINK1) targets mitochondria for its degradation through autophagy.

Physical form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Hector Flavio Ortega-Arellano et al.
Neurochemical research, 44(8), 1986-1998 (2019-07-17)
Parkinson's disease (PD) is a progressive neurodegenerative disorder associated with oxidative stress. Therefore, finding new antioxidant sources might be beneficial for its treatment. Avocado Persea americana is a fruit widely cultivated in tropical and subtropical climates worldwide. Although avocado by-products...
Cristina Ottone et al.
Gene, 470(1-2), 12-19 (2010-09-28)
Mutations in the human parkin (PARK2) gene cause autosomal recessive-juvenile Parkinson's disease (AR-JP). In Drosophila melanogaster, mutant parkin alleles display a broad range of phenotypic alterations, including female infertility. Here we report that reducing the level of eukaryotic translation initiation...
Hector Flavio Ortega-Arellano et al.
Neurotoxicology, 60, 42-53 (2017-03-13)
Autosomal recessive Juvenile Parkinsonism (AR-JP) is a chronic, progressive neurodegenerative disorder caused by mutation in the PARKIN gene, and invariably associated with dopaminergic (DAergic) neuronal loss and brain iron accumulation. Since current medical therapy is symptomatic and lacks significant disease-modifying...
P Githure M'Angale et al.
Genome, 60(3), 241-247 (2017-01-21)
Mutations in parkin (PARK2) and Pink1 (PARK6) are responsible for autosomal recessive forms of early onset Parkinson's disease (PD). Attributed to the failure of neurons to clear dysfunctional mitochondria, loss of gene expression leads to loss of nigrostriatal neurons. The...
Zong-Heng Wang et al.
Human molecular genetics, 25(10), 1946-1964 (2016-03-05)
PINK1/Parkin-mediated mitochondrial quality control (MQC) requires valosin-containing protein (VCP)-dependent Mitofusin/Marf degradation to prevent damaged organelles from fusing with the healthy mitochondrial pool, facilitating mitochondrial clearance by autophagy. Drosophila clueless (clu) was found to interact genetically with PINK1 and parkin to...

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