SAB1300403

Sigma-Aldrich

Anti-PARG (C-term) antibody produced in rabbit

saturated ammonium sulfate (SAS) precipitated, buffered aqueous solution

Synonym(s):
Anti-Poly(ADP-ribose) glycohydrolase
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

saturated ammonium sulfate (SAS) precipitated

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

application(s)

immunohistochemistry: 1:50-1:100
indirect ELISA: 1:1000
western blot: 1:100-1:500

conjugate

unconjugated

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... PARG(8505)

General description

PARG (Poly ADP-ribose glycohydrolase) is a catabolic enzyme with molecular mass ranging from 60-74kDa. It has four isoforms namely G1, G2, G3 and G4 with high protease sensitivity.

Immunogen

PARG (NP_003622, 395-431)
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Parg.

Application

Anti-PARG (C-term) antibody produced in rabbit is suitable for immunohistochemistry (1:50-1:100),indirect ELISA (1:1000) and western blot (1:100-1:500) applications.

Biochem/physiol Actions

PARG (Poly ADP-ribose glycohydrolase) is involved in several gene regulation processes such as maintenance of chromatin structure, transcription, DNA repair and apoptosis. It regulates retinoic acid receptor (RAR)-mediated transcription during chromatin remodeling. It catabolically separate ADP-ribose polymers synthesized by poly-(ADP-ribose) polymerases. A study shows that PARG plays a therapeutic role in various physiological conditions such as inflammation, ischemia, stroke, and cancer chemotherapy.

Physical form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Wookee Min et al.
Frontiers in bioscience (Landmark edition), 14, 1619-1626 (2009-03-11)
Poly (ADP-robose) glycohydrolase (PARG) is a catabolic enzyme that cleaves ADP-ribose polymers synthesized by members of the poly (ADP-ribose) polymerase (PARP) family of enzymes. The growing evidence supports the importance of a tight control of poly (ADP-ribose) metabolism by the...
M-E Bonicalzi et al.
Cellular and molecular life sciences : CMLS, 62(7-8), 739-750 (2005-05-04)
Poly(ADP-ribose) glycohydrolase (PARG) is a catabolic enzyme that cleaves ADP-ribose polymers formed by members of the PARP family of enzymes. Despite its discovery and subsequent partial purification in the 1970s and the cloning of its single gene in the late...
Nicolas Le May et al.
Molecular cell, 48(5), 785-798 (2012-10-30)
Poly-(ADP-ribose) glycohydrolase (PARG) is a catabolic enzyme that cleaves ADP-ribose polymers synthesized by poly-(ADP-ribose) polymerases. Here, transcriptome profiling and differentiation assay revealed a requirement of PARG for retinoic acid receptor (RAR)-mediated transcription. Mechanistically, PARG accumulates early at promoters of RAR-responsive...

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