SAB1408000

Sigma-Aldrich

Anti-GRHL2 antibody produced in mouse

purified immunoglobulin, buffered aqueous solution

Synonym(s):
MGC149294, FLJ11172, MGC149295, BOM, DFNA28, FLJ13782, TFCP2L3
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~71.1 kDa

species reactivity

human

application(s)

indirect immunofluorescence: suitable
western blot: 1 μg/mL

conjugate

unconjugated

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... GRHL2(79977)

General description

Grainyhead like transcription factor 2 (GRHL2) encodes a transcription factor that can act as a homodimer or as a heterodimer with either GRHL1 or GRHL3. Defects in this gene are a cause of non-syndromic sensorineural deafness autosomal dominant type 28 (DFNA28). Grainyhead like transcription factor 2 (GRHL2) is an epithelial regulatory factor. GRHL2 is homologous to Drosophila grainyhead. GRHL2 gene is located on human chromosome 8q22.3.

Immunogen

GRHL2 (NP_079191.1, 1 a.a. ~ 625 a.a) full-length human protein.

Sequence
MSQESDNNKRLVALVPMPSDPPFNTRRAYTSEDEAWKSYLENPLTAATKAMMSINGDEDSAAALGLLYDYYKVPRDKRLLSVSKASDSQEDQEKRNCLGTSEAQSNLSGGENRVQVLKTVPVNLSLNQDHLENSKREQYSISFPESSAIIPVSGITVVKAEDFTPVFMAPPVHYPRGDGEEQRVVIFEQTQYDVPSLATHSAYLKDDQRSTPDSTYSESFKDAATEKFRSASVGAEEYMYDQTSSGTFQYTLEATKSLRQKQGEGPMTYLNKGQFYAITLSETGDNKCFRHPISKVRSVVMVVFSEDKNRDEQLKYWKYWHSRQHTAKQRVLDIADYKESFNTIGNIEEIAYNAVSFTWDVNEEAKIFITVNCLSTDFSSQKGVKGLPLMIQIDTYSYNNRSNKPIHRAYCQIKVFCDKGAERKIRDEERKQNRKKGKGQASQTQCNSSSDGKLAAIPLQKKSDITYFKTMPDLHSQPVLFIPDVHFANLQRTGQVYYNTDDEREGGSVLVKRMFRPMEEEFGPVPSKQMKEEGTKRVLLYVRKETDDVFDALMLKSPTVKGLMEAISEKYGLPVEKIAKLYKKSKKGILVNMDDNIIEHYSNEDTFILNMESMVEGFKVTLMEI

Application

Anti-GRHL2 antibody produced in mouse has been used in:
  • Immunofluorescence
  • Western blotting
  • Co-immunoprecipitation
  • Immunohistochemistry

Biochem/physiol Actions

Grainyhead like transcription factor 2 (GRHL2) inhibits epithelial-to-mesenchymal transition, which is necessary for cell migration. It is involved in neural tube closure, epithelial morphogenesis and barrier formation during embryogenesis. It contributes to carcinogenesis. GRHL2 regulates epithelial plasticity of pancreatic ductal adenocarcinoma (PDAC) progression.

Physical form

Solution in phosphate buffered saline, pH 7.4

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Grhl2 regulation of SPINT1 expression controls salivary gland development
Matsushita T, et al.
Biochemical and Biophysical Research Communications, 137(22) (2018)
Grainyhead-like 2 (GRHL 2) regulates epithelial plasticity in pancreatic cancer progression
Nishino H, et al.
Cancer Medicine, 6(11) (2017)
Novel androgen receptor co-regulator GRHL2 exerts both oncogenic and anti-metastatic functions in prostate cancer
Paltoglou S, et al.
Cancer Research, 137(22) (2017)
Grainyhead-like 2 in development and cancer
Ma L, et al.
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine, 39(5), 1010428317698375-1010428317698375 (2017)
The transcription factor grainyhead-like 2 regulates the molecular composition of the epithelial apical junctional complex
Werth M, et al.
Development, 137(22), 3835-3845 (2010)

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