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SAB2702167

Sigma-Aldrich

Monoclonal Anti-TSG101 antibody produced in mouse

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Synonym(s):
TSG10, VPS23
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4A10
monoclonal

form

liquid

mol wt

46 kDa

species reactivity

human, monkey, mouse, rat, hamster

concentration

1 mg/mL

technique(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1:500-1:3000

isotype

IgG1

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TSG101(7251)

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This Item
WH0007251M1T5701T5826
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

rabbit

clone

4A10, monoclonal

clone

5B7, monoclonal

clone

polyclonal

clone

polyclonal

Gene Information

human ... TSG101(7251)

Gene Information

human ... TSG101(7251)

Gene Information

human ... TSG101(7251)
mouse ... Tsg101(22088)
rat ... Tsg101(292925)

Gene Information

human ... TSG101(7251)
mouse ... Tsg101(22088)
rat ... Tsg101(292925)

species reactivity

human, monkey, mouse, rat, hamster

species reactivity

human

species reactivity

rat, mouse, human

species reactivity

mouse, human, rat

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General description

Tumor susceptibility gene 101 (TSG101) is an endosomal sorting complex required for transport (ESCRT) component, present on endosomal membranes. This gene is located on chromosome 11p15. The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a leucine-zipper motif within a coiled-coil domain near the carboxyl terminus.

Immunogen

Amino acids 167-374 of TSG101 protein expressed in E. coli.

Application

Suggested starting dilutions are as follows: WB: 1:500-1:3000. Suggested fixation for ICC/IF: 3-3.5% PFA with 0.05% saponin treatment. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Monoclonal Anti-TSG101 antibody has been used in western blotting and immunoprecipitation (IP) analyses.

Biochem/physiol Actions

The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a luecine-zipper motif within a coiled-coil domain near the carboxyl terminus.
Tumor susceptibility gene 101 (TSG101) is essential for the generation of central supramolecular activation cluster, and the signal termination of T cell receptor (TCR) microclusters (MC). It plays an important role in TCR downregulation and separation of TCR- major histocompatibility complex (MHC)-peptide from signaling complexes, that are enriched with protein kinase C θ (PKCθ).
TSG101-4A10 recognizes TSG101 protein, the product of a recently identified tumor susceptibility gene whose inactivation in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Phosphate-buffered saline, no preservative added.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Bettina Mannerström et al.
Epigenetics, 14(4), 352-364 (2019-03-26)
Extracellular vesicles (EVs) are central to intercellular communication and play an important role in cancer progression and development. Osteosarcoma (OS) is an aggressive bone tumour, characterized by the presence of malignant mesenchymal cells. The specific tumour-driving genetic alterations that are
Absence of TSG101 transcript abnormalities in human cancers.
Trink B, et al.
Oncogene, 16(21), 2815-2815 (1998)
Bone marrow niche trafficking of miR-126 controls the self-renewal of leukemia stem cells in chronic myelogenous leukemia.
Zhang B, et al.
Nature Medicine, 24(4), 450-450 (2018)
Essential role of ubiquitin and TSG101 protein in formation and function of the central supramolecular activation cluster.
Vardhana S, et al.
Immunity, 32(4), 531-540 (2010)
Shengqiang Xu et al.
ACS biomaterials science & engineering, 4(2), 654-662 (2018-04-07)
MicroRNA (miRNA) in urine has been considered as a potential biomarker for early-stage diagnosis of multiple diseases like urinary system cancer, kidney injury and diabetes, owing to their many demonstrated advantages including long-term stability and noninvasiveness. However, the traditional enrichment

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