SAB2702364

Monoclonal Anti-Iba1 antibody produced in mouse

clone GT10312, affinity isolated antibody

• Synonym(s): AIF-1, IBA1, IRT-1, IRT1
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: GT10312, monoclonal
• form: buffered aqueous solution
• mol wt: 17kDa
• species reactivity: mouse, human, rat
• concentration: 2.03mg/mL
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:1000; indirect immunofluorescence: suitable; western blot: 500-3000
• isotype: IgG1
• UniProt accession no.: P55008
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... AIF1(199)

Description

General description

Ionized calcium-binding adapter molecule 1 (IBA1), also called the allograft inflammatory factor 1 (AIF1) is mapped to human chromosome 6p21.33. It encodes a 17 kDa protein and comprises calcium binding EF hand motif. IBA1 is expressed in lung, tonsil, lymph node and spleen. In addition,it is also expressed in macrophages and glial cells.

Immunogen

Recombinant protein encompassing a sequence within the center region of human Iba1.

Application

Monoclonal Anti-Iba1 antibody produced in mouse has been used:

• in the double immunofluorescence
• in the immunofluorescence
• in immunohistochemistry

Biochem/physiol Actions

Ionized calcium-binding adapter molecule 1 (IBA1) interacts with filamentous actin (F‐actin) and L‐fimbrin. Its expression is modulated during injury. Inflammatory cytokines also induce IBA1 expression. IBA1 expression is implicated in the graft rejection in coronary artery vasculopathy (CAV). It is highly expressed in hepatocellular carcinoma and may serve a potential marker and therapeutic target. Increased expression of AIF1 in synovial fluid and peripheral blood monocytes is implicated in rheumatoid arthritis.

Features and Benefits

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Other Notes

Purification: Affinity purified by Protein G

Physical form

Phosphate-buffered saline, no preservative added.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable