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SAB2702364

Sigma-Aldrich

Monoclonal Anti-Iba1 antibody produced in mouse

clone GT10312, affinity isolated antibody

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Synonym(s):
AIF-1, IBA1, IRT-1, IRT1
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

GT10312, monoclonal

form

buffered aqueous solution

mol wt

17kDa

species reactivity

mouse, human, rat

concentration

2.03mg/mL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:1000
indirect immunofluorescence: suitable
western blot: 500-3000

isotype

IgG1

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... AIF1(199)

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SAB2500042MABN92HPA062949
vibrant-m

MABN92

Anti-Iba1/AIF1 Antibody

species reactivity

mouse, human, rat

species reactivity

mouse, rat, human

species reactivity

rat, mouse, human

species reactivity

human

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

biological source

mouse

biological source

goat

biological source

mouse

biological source

rabbit

clone

GT10312, monoclonal

clone

polyclonal

clone

20A12.1, monoclonal

clone

polyclonal

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

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General description

Ionized calcium-binding adapter molecule 1 (IBA1), also called the allograft inflammatory factor 1 (AIF1) is mapped to human chromosome 6p21.33. It encodes a 17 kDa protein and comprises calcium binding EF hand motif. IBA1 is expressed in lung, tonsil, lymph node and spleen. In addition,it is also expressed in macrophages and glial cells.

Immunogen

Recombinant protein encompassing a sequence within the center region of human Iba1.

Application

Monoclonal Anti-Iba1 antibody produced in mouse has been used:
  • in the double immunofluorescence
  • in the immunofluorescence
  • in immunohistochemistry

Biochem/physiol Actions

Ionized calcium-binding adapter molecule 1 (IBA1) interacts with filamentous actin (F‐actin) and L‐fimbrin. Its expression is modulated during injury. Inflammatory cytokines also induce IBA1 expression. IBA1 expression is implicated in the graft rejection in coronary artery vasculopathy (CAV). It is highly expressed in hepatocellular carcinoma and may serve a potential marker and therapeutic target. Increased expression of AIF1 in synovial fluid and peripheral blood monocytes is implicated in rheumatoid arthritis.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Other Notes

Purification: Affinity purified by Protein G

Physical form

Phosphate-buffered saline, no preservative added.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Expression of allograft inflammatory factor-1 in peripheral blood monocytes and synovial membranes in patients with rheumatoid arthritis
Pawlik A, et al.
Human immunology, 77(1), 131-136 (2016)
Treadmill exercise decreases Abeta deposition and counteracts cognitive decline in APP/PS1 mice, possibly via hippocampal microglia modifications
Zhang X, et al.
Frontiers in Aging Neuroscience, 11, 78-78 (2019)
Structural and functional characterization of human Iba proteins
Schulze JO, et al.
The FEBS journal, 275(18), 4627-4640 (2008)
Xianliang Zhang et al.
Frontiers in aging neuroscience, 11, 78-78 (2019-04-27)
Recent studies have suggested that exercise may be beneficial for delaying or attenuating Alzheimer's disease (AD). However, the underlying mechanisms were not clear. Microglia-mediated neuroinflammation is suggested to play an important role in the pathology of AD. The present study
Expression of Allograft Inflammatory Factor-1 (AIF-1) in Hepatocellular Carcinoma
Zhang Q, et al.
Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 24, 6218-6218 (2018)

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