SAB3500082

Sigma-Aldrich

Anti-NOTUM antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-NOTUM pectinacetylesterase homolog
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human, rat, mouse

application(s)

immunofluorescence: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable

conjugate

unconjugated

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... NOTUM(147111)

General description

NOTUM encodes palmitoleoyl-protein carboxylesterase. In human chromosome, the gene NOTUM is localized on 17q25.3.

Immunogen

NOTUM antibody was raised against a 19 amino acid peptide near the amino terminus of human NOTUM.

Application

Anti-NOTUM antibody produced in rabbit has been used in immunostaining.

Biochem/physiol Actions

Notum is involved in the progression of colorectal cancer and its expression levels is higher in metastatic cells. Notum is one of the pain associated genes.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Target description

In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by β-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway.

Linkage

The action of this antibody can be blocked using blocking peptide SBP3500082.

Physical form

Supplied at approx. 1 mg/mL in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
null
Establishment of a murine pancreatic cancer pain model and microarray analysis of pain-associated genes in the spinal cord dorsal horn
Wang L, et al.
Molecular Medicine Reports, 16(4), 4429-4436 (2017)
Transcriptional dynamics of cultured human villous cytotrophoblasts
Robinson JF, et al.
Endocrinology, 158(6), 1581-1594 (2017)
NOTUM Is Involved in the Progression of Colorectal Cancer
Yoon JH, et al.
Cancer, Genomics, and Proteomics, 15(6), 485-497 (2018)
Joshua F Robinson et al.
Endocrinology, 158(6), 1581-1594 (2017-03-23)
During human pregnancy, cytotrophoblasts (CTBs) play key roles in uterine invasion, vascular remodeling, and anchoring of the feto-placental unit. Due to the challenges associated with studying human placentation in utero, cultured primary villous CTBs are used as a model of...

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