All Photos(3)

SAB4100599

Sigma-Aldrich

Anti-KRAS antibody, Mouse monoclonal

clone KRAS-A582, purified from hybridoma cell culture

Synonym(s):
Anti-K-RAS4B, Anti-K-RAS2A, Anti-K-RAS4A, Anti-C-K-RAS, Anti-K-RAS2B

Quality Level

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

KRAS-A582, monoclonal

form

aqueous solution

mol wt

antigen predicted mol wt 27.9 kDa

species reactivity

human

application(s)

western blot: suitable using 1:250-1:500 using HEK-293 cells expressing KRAS

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... KRAS(3845)

General description

The kirsten rat sarcoma-2 virus oncogene (KRAS) gene is mapped to human chromosome 12p12.1 and belongs to the family of Ras genes. The protein encoded is a small guanosine nucleotide-bound GTPases belonging.

Immunogen

KRAS purified recombinant protein

Biochem/physiol Actions

Mutation in the KRAS gene causes non small-cell lung cancer and promotes tumor initiation, progression and metastasis. Altered KRAS gene also offers resistance to chemotherapy. It is involved in the signal transduction between epidermal growth factor receptor (EGFR) to the nucleus.

Physical form

Solution in Dulbecco′s Modified Eagle′s Medium with 10% fetal calf serum and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

RAS-expanded mutations and HER2 expression in metastatic colorectal cancer: a new step of precision medicine
Valentini AM, et al.
Applied Immunohistochemistry & Molecular Morphology, 26(8), 539-539 (2018)
Restoration of mutant K-Ras repressed miR-199b inhibits K-Ras mutant non-small cell lung cancer progression
Jin H, et al.
Journal of Experimental & Clinical Cancer Research, 38(1), 165-165 (2019)
The RASopathies: developmental syndromes of Ras/MAPK pathway dysregulation
Tidyman WE and Rauen KA
Current Opinion in Genetics & Development, 19(3), 230-236 (2009)
Wulamujiang Aini et al.
Transplant immunology, 31(2), 55-59 (2014-07-06)
In living donor liver transplantation, the biological organ age of the donated allograft is unknown in young patients who receive grafts from older donors. Few studies have focused on the effects of aging on allografts in the state of tolerance....
Hua Yang et al.
Anti-cancer drugs, 25(7), 767-777 (2014-04-02)
Histone deacetylase inhibitors are a new class of anticancer agents that inhibit cancer cell proliferation and induce apoptosis and cell cycle arrest in various cancer cells. Recently, we identified ZYJ-34c, a modified histone deacetylase inhibitor that showed significantly higher antitumor...

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