MilliporeSigma
All Photos(1)

SAB4200013

Sigma-Aldrich

Anti-Kindlin-3 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-MIG-2, Anti-Fermitin family homolog 3, Anti-KIND3, Anti-MIG2B, Anti-URP2SF, Anti-FERMT3, Anti-UNC-112 related protein 2, Anti-UNC112C, Anti-URP2
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~72 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

western blot: 5-10 μg/mL using whole extracts of human TF1 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FERMT3(83706)

General description

Kindlin-3 is a member of the kindlin family of focal adhesion proteins. The kindlin family consists of three members in vertebrates, kindlin-1, kindlin-2 and kindlin-3 that share high sequence homology and a common structure containing a C-terminal 4.1R, ezrin, radixin, moesin (FERM) domain and a pleckstrin-homology domain. Kindlin-1 and kindlin-2 are widely expressed, kindlin-3 is preferentially expressed in hematopoietic cells, mainly in megakaryocytes and platelets.

Application

Anti-Kindlin-3 antibody produced in rabbit has been used in western blotting and immunoblotting.

Biochem/physiol Actions

Kindlin-3 is required for integrin-mediated leukocyte adhesion to endothelial cells. Mutations in kindlin-3 cause leukocyte adhesion deficiency-III. Kindlin-1, kindlin-2 and kindlin-3 are essential for the regulation of integrin activation by binding the tails of integrin-β subunits. Loss of kindlins results in severe defects in integrin signaling, cell-ECM adhesion and cytoskeletal organization. Deficiency of kindlin-1 results in kindler syndrome, kindlin-2 results in early embryonic lethality due to cardiac developmental problems, and kindlin-3 results in postnatal lethality.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Kindling the flame of integrin activation and function with kindlins
Plow EF, et al.
Current Opinion in Hematology, 16(5), 323-323 (2009)
Peripheral blood mononuclear cell proteome changes in patients with myelodysplastic syndrome
Pecankova K, et al.
BioMed Research International, 2015(3), 325-325 (2015)
Kindlins: essential regulators of integrin signalling and cell-matrix adhesion
Larjava H, et al.
EMBO Reports, 9(12), 1203-1208 (2008)
Kindlin-3 is essential for integrin activation and platelet aggregation
Moser M, et al.
Nature Medicine, 14(3), 325-325 (2008)
The Kindlins: subcellular localization and expression during murine development
Ussar S, et al.
Experimental Cell Research, 312(16), 3142-3151 (2006)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service