SAB4200023

Sigma-Aldrich

Anti-VPS4B antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-SKD2, Anti-SKD1, Anti-SKD1-homolog, Anti-VPS4-1, Anti-Vacuolar protein sorting factor 4A, Anti-VPS4, Anti-Vacuolar protein sorting 4 homolog A, Anti-SKD1A

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

mouse, human

concentration

~1.0 mg/mL

application(s)

immunoprecipitation (IP): 10-20 μg using lysate of mouse 3T3 cells
western blot: 2-4 μg/mL using whole extracts of G361 cells

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... VPS4B(9525)

General description

Vacuolar protein sorting 4 homolog B (Vps4B) is a member of the AAA protein family (ATPases associated with diverse cellular activities) and is a homolog of yeast Vps4 protein. In humans, two of yeast protein paralogs have been identified as Vps4A and Vps4B. Both share high degree of amino acid sequence. The gene for Vps4B resides on human chromosome 18.

Application

Anti-VPS4B antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Vacuolar protein sorting 4 (Vps4) mediates endosomal membrane protein sorting, retroviral budding, and cytokinesis by recognizing membrane-associated endosomal sorting complexes required for transport (ESCRT-III) assemblies and catalysing their disassembly.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Luana L Scheffer et al.
Nature communications, 5, 5646-5646 (2014-12-24)
In muscle and other mechanically active tissue, cell membranes are constantly injured, and their repair depends on the injury-induced increase in cytosolic calcium. Here, we show that injury-triggered Ca(2+) increase results in assembly of ESCRT III and accessory proteins at...
Comparative sequence and expression analyses of four mammalian VPS4 genes
Beyer A, et al.
Gene, 305(1), 47-59 (2003)
Membrane scission by the ESCRT-III complex
Wollert T, et al.
Nature, 458(7235), 172-172 (2009)

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