SAB4200033

Sigma-Aldrich

Anti-BMI1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-Polycomb group RING finger protein 4, Anti-RNF51, Anti-PCGF4, Anti-B lymphoma Mo-MLV insertion region 1 homolog; ring finger protein 51
NACRES:
NA.41

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~37 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

application(s)

indirect immunofluorescence: 1-2 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human BMI1
western blot: 1-2 μg/mL using lysate of HEK-293T cells over expressing human BMI1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... BMI1(648)
mouse ... Bmi1(12151)
rat ... Bmi1(307151)

General description

The human proto-oncogene BMI1 (also known as Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, and RING finger protein 51) is a member of the mammalian Polycomb group (Pc-G) genes.

Application

Anti-BMI1 antibody produced in rabbit may be used in several immunochemical techniques including immunoblotting (~37 kDa) and immunofluorescence.

Biochem/physiol Actions

BMI1 (Proto-Oncogene, Polycomb Ring Finger) plays an important role as epigenetic gene silencers during development. Elevated expression of BMI1 is associated with many cancers such as mantle cell lymphoma, B-cell non-Hodgkin′s lymphoma, myeloid leukemia, non-small cell lung cancer, colorectal cancer, breast cancer and prostate cancer. BMI1 has been shown to be also required for self-renewal of hematopoietic stem cells and neuronal stem cells. BMI1 knockout in mice results in defects in hematopoiesis, skeletal patterning and neurological functions.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Bmi-1 cooperates with H-Ras to transform human mammary epithelial cells via dysregulation of multiple growth-regulatory pathways
Datta S, et al.
Cancer Research, 67(21), 10286-10295 (2007)
Bmi-1 is required for maintenance of adult self-renewing haematopoietic stem cells
Park I K, et al.
Nature, 423(6937), 302-302 (2003)
Bmi1, stem cells, and senescence regulation
Park IK, et al.
The Journal of Clinical Investigation, 113(2), 175-179 (2004)
Bmi-1 dependence distinguishes neural stem cell self-renewal from progenitor proliferation
Molofsky AV, et al.
Nature, 425(6961), 962-962 (2003)
Hsiao-Fan Chen et al.
Nature communications, 5, 4697-4697 (2014-08-26)
The mechanisms controlling tumour-induced angiogenesis are presently not clear. In principle, angiogenesis can be achieved through the activation of endothelial cells in existing vessels or by transdifferentiation of tumour cells into endothelial cells. However, whether tumour cells can go through...

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