SAB4200049

Sigma-Aldrich

Anti-FRAT1 (C-terminal) antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody

Synonym(s):
Anti-Frequently rearranged in advanced T-cell lymphomas, Anti-GBP
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

mol wt

antigen ~29 kDa

species reactivity

human, rat

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.5 mg/mL

application(s)

western blot: 1.5-3 μg/mL using HEK-293T cell extract over expressing human FRAT1 and a rat skeletal muscle extract (S1 fraction)

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FRAT1(10023)

General description

Frequently rearranged in advanced T-cell lymphomas-1 (FRAT1) is a glycogen synthase kinase 3 β (GSK3β)-binding protein and belongs to the FRAT family. This protein contains a conserved GSK3β interacting domain. The FRAT1 gene is ubiquitously expressed and is mapped on the human chromosome at 10q24.1.

Specificity

Anti-FRAT1 (C-terminal) specifically recognizes human and rat FRAT1.

Application

Anti-FRAT1 (C-terminal) antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

Frequently rearranged in advanced T-cell lymphomas-1 (FRAT1) protein plays a pivotal role in positively regulating the wingless-related integration site (Wnt)/β-catenin signaling pathway. This protein competes with axin for binding of glycogen synthase kinase 3 β (GSK3β), thus displacing GSK3β from the axin-β-catenin complex. The FRAT1 gene expression is upregulated in several human cancer lines primarily in gastric cancer. Overexpression of this gene is associated with esophageal squamous cell carcinoma (ESCC), ovarian, breast and cervical carcinoma. The FRAT1 gene expression is also observed in astrocytomas and human gliomas.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Yong Zhang et al.
Virchows Archiv : an international journal of pathology, 459(3), 255-263 (2011-08-06)
Frat1 has been reported to be overexpressed in several human malignant tumors, including esophageal squamous, cervical, breast, and ovarian carcinoma, but the role of Frat1 in lung cancer is unknown. Our purpose is to investigate the expression of Frat1 and...
Tetsuroh Saitoh et al.
International journal of oncology, 20(4), 785-789 (2002-03-15)
FRAT1 and FRAT2 genes, clustered in human chromosome 10q24, are human homologues to mouse proto-oncogene Frat1, which promotes carcinogenesis through activation of the WNT - beta-catenin - TCF signaling pathway. FRAT1 and FRAT2 mRNAs are up-regulated together in a gastric...
Yihua Wang et al.
International journal of cancer, 123(3), 561-568 (2008-05-24)
Esophageal squamous cell carcinoma (ESCC) is an aggressive tumor with a poor prognosis. Although aberrant activation of beta-catenin/T-cell factor (TCF) pathway has been observed in ESCC, mechanisms underlying this phenomenon remain unknown. Frequently rearranged in advanced T-cell lymphomas-1 (FRAT1), overexpressed...

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