SAB4200085

Monoclonal Anti-AGO2 antibody produced in rat

~1.5 mg/mL, clone 11A9, purified immunoglobulin

• Synonym(s): AGO2 Antibody - Monoclonal Anti-AGO2 antibody produced in rat, Ago2 Antibody, Anti-Argonaute 2, Anti-EIF2C2 eukaryotic translation initiation factor 2C, 2, Anti-Q10
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 11A9, monoclonal
• Application: ICC, IP, WB
• Citations: 72

Properties

• biological source: rat
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 11A9, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~85 kDa
• species reactivity: monkey, rat, human, mouse, bovine
• packaging: antibody small pack of 25 μL
• concentration: ~1.5 mg/mL
• technique(s): immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 0.5-1.0 μg/mL using HeLa cell extracts
• isotype: IgG2a
• UniProt accession no.: Q9UKV8
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Quality Level: 200
• Gene Information: human ... AGO2(27161) ; rat ... Ago2(59117)

Description

General description

Monoclonal Anti-AGO2 (rat IgG2a isotype) is derived from the hybridoma 11A9 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with a peptide corresponding to amino acids 1-15 of human AGO2. Monoclonal Anti-AGO2 recognizes human, monkey, bovine, rat and mouse AGO2. Argonaute 2 (Ago2) is encoded by the gene mapped to human chromosome 8q24.3. The encoded protein belongs to the Ago protein family. AGO2 is mainly localized on the diffuse cytoplasm and stress granules.

Application

Monoclonal Anti-AGO2 antibody produced in rat has been used in:

• immunofluorescent localization
• western blot analysis[1][2]
• immunoprecipitation.[3][1]

Biochem/physiol Actions

Argonaute 2 (Ago2) plays a vital role in short interfering RNA-mediated gene silencing. The encoded protein is regulated at both the transcriptional and posttranslational level in human breast cancer cell lines. Ago2 and enhanced micro-RNA activity is involved in the tumorigenic progression of breast cancer cell lines. Overexpression of the gene has been observed in hepatocellular carcinoma (HCC), head and neck squamous cell carcinoma patients.

Physical form

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Preparation Note

Store at –20°C. For continuous use, the product may be stored at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Other Notes

Cell lysates should not be boiled before loading onto SDS-PAGE, but only warmed to 60°C for 15 min. In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable