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SAB4200195

Sigma-Aldrich

Anti-Brg1 antibody, Rat monoclonal

clone 5B7, purified from hybridoma cell culture

Synonym(s):

Anti-BAF190, Anti-RTPS2, Anti-SMARCA4, Anti-SNF2, Anti-SNF2-BETA, Anti-SNF2L4, Anti-SNF2LB, Anti-SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4, Anti-SWI2, Anti-hSNF2b, Monoclonal Anti-Brg1 antibody produced in rat

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rat

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

5B7, monoclonal

form

buffered aqueous solution

mol wt

antigen ~185 kDa

species reactivity

mouse, monkey, human

concentration

~1.0 mg/mL

technique(s)

ChIP: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.5-1.0 μg/mL using HeLa, HEK-293T, Raji or HepG2 cells extracts

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SMARCA4(6597)
mouse ... Smarca4(20586)

General description

Brahma-related gene 1 (Brg1), also known as SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), is encoded by the gene mapped to human chromosome 19p13.2. The encoded protein is characterized with a conserved catalytic ATPase domain, a conserved C-terminal bromodomain, AT-hook motif and the less characterized N-terminal region housing QLQ, helicase-SANT-associated (HSA) and BRM and KIS (BRK) domains.
Monoclonal Anti-Brg1 (rat IgG2a isotype) is derived from the hybridoma 5B7 produced by the fusion of mouse myeloma cells (SP2) and splenocytes from rat immunized with a fusion protein corresponding to a fragment of human Brg1. Brahma-related gene 1 (Brg1) catalytic subunit of mammalian SWItch/sucrose non-fermentable (SWI/SNF)-related complexes co-localizes with origin recognition complexes, GINS complexes and proliferating cell nuclear antigen.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Monoclonal Anti-Brg1 antibody produced in rat has been used in:
  • immunoblotting
  • immunocytochemistry
  • immunoprecipitation
  • chromatin immunoprecipitation

Biochem/physiol Actions

Brahma-related gene 1 (Brg1) acts as a transcriptional repressor. It interacts with retinoblastoma tumor suppressor to form a repressor complex which inhibits cell cycle proteins such as cyclins A, D1 and E. Brg1 plays an important role as tumor suppressor to maintain genome stability and prevent cancer.
Brahma-related gene 1 (Brg1), acts as a catalytic subunit for various mammalian SWItch/Sucrose non-fermentable (SWI/SNF) complexes, where it helps in transcription regulation through the nucleosome remodelling. Additionally, it plays an essential role in differentiation of myeloid to granulocytes. Mutation in the gene is associated with the development of Coffin-Siris syndrome and rhabdoid tumor predisposition syndrome. Loss of Brg1 expression is used as a potential diagnostic marker for ovarian small cell carcinoma of the hypercalcemic type (SCCOHT).

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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The BRG1 transcriptional coregulator
Trotter KW and Archer TK.
Nuclear Receptor Signaling (2008)
Germline nonsense mutation and somatic inactivation of SMARCA4/BRG1 in a family with rhabdoid tumor predisposition syndrome.
Schneppenheim R
American Journal of Human Genetics, 86, 279-284 (2010)
SMARCA4 (BRG1) loss of expression is a useful marker for the diagnosis of ovarian small cell carcinoma of the hypercalcemic type (ovarian rhabdoid tumor): a comprehensive analysis of 116 rare gynecologic tumors, 9 soft tissue tumors, and 9 melanomas.
Karanian-Philippe M
American Journal of Surgical Pathology, 39, 1197-1205 (2015)
Mutations affecting components of the SWI/SNF complex cause Coffin-Siris syndrome.
Tsurusaki Y
Nature Genetics, 44, 376-378 (2012)
Brg1, the ATPase subunit of the SWI/SNF chromatin remodeling complex, is required for myeloid differentiation to granulocytes.
Vradii D
Journal of Cellular Physiology, 206, 112-118 (2006)

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