MilliporeSigma
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SAB4200283

Sigma-Aldrich

Anti-Tensin 1 antibody produced in rabbit

enhanced validation

~1.0 mg/mL, affinity isolated antibody

Synonym(s):
Anti-TNS1
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 225 kDa (rat)
antigen ~150 kDa (human)

species reactivity

rat, chicken, human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.0 mg/mL

technique(s)

immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded rat heart.
indirect immunofluorescence: 3-6 μg/mL using chicken embryo fibroblast cells.
western blot: 1-2 μg/mL using lysates of HEK-293T cells overexpressing human tensin 1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TNS1(7145)
mouse ... Tns1(21961)
rat ... Tns1(301509)

General description

Tensin 1 (TNS1) is encoded by the gene mapped to human chromosome 2q35. The encoded protein is localized to focal adhesions and is mainly expressed in renal tubular epithelial cells (TECs).

Immunogen

synthetic peptide corresponding to a sequence located in the internal region of human tensin1, conjugated to KLH. The corresponding sequence is identical in rat and mouse tensin1.

Application

Anti-Tensin 1 antibody may be used in immunofluorescence and immunohistochemistry.
Anti-Tensin 1 antibody produced in rabbit has been used in immunostaining and immunoblotting.

Biochem/physiol Actions

Tensin 1 associates with actin, integrin, FAK, p130Cas, DLC-1 and protein phosphatase PP1 which affects cell polarization, migration and invasion. Tensin 1 undergoes extensive Ser/Thr and Tyr phosphorylation induced by extracellular matrix (ECM), various growth factors and oncogenes. In addition, tensin 1 is cleaved by calpain II, a focal adhesion protease, suggesting that tensin 1 plays important roles in organizing the actin cytoskeleton and mediating signal transduction.
Tensin 1 (TNS1) contributes to transforming growth factor (TGF)-β-induced myofibroblast differentiation and extracellular fibronectin and collagen matrix formation. Disruption in the gene and associated signaling might hinder tissue fibrosis. The encoded protein anchors to stress fibers by interacting with cytoplasmic tails of integrins and increases metastatic abilities of cancer cells. Tensin plays a vital role in activation of the c-Jun N-terminal kinases (JNK) and p38 pathways.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Tensin is potentially involved in extracellular matrix production in mesangial cells.
Yamashita M
Histochemistry and Cell Biology, 121, 245-254 (2004)
Genetic association analyses highlight biological pathways underlying mitral valve prolapse
Dina C
Nature Genetics, 47, 1206?1211-1206?1211 (2015)
Tensin
Lo S H
The International Journal of Biochemistry & Cell Biology, 36(1), 31-34 (2004)
Tensin 1 Is Essential for Myofibroblast Differentiation and Extracellular Matrix Formation.
Bernau K
American Journal of Respiratory Cell and Molecular Biology, 56, 465-476 (2017)
Tensin can induce JNK and p38 activation.
Katz BZ
Biochemical and Biophysical Research Communications, 272, 717-720 (2000)

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