SAB4200391

Sigma-Aldrich

Anti-HIP1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):
Anti-Huntingtin interacting protein 1
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~116 kDa

concentration

~1.0 mg/mL

application(s)

western blot: 1-2 μg/mL using mouse and rat brain extracts (S1 fraction).

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

Gene Information

mouse ... Hip1(215114)
rat ... Hip1(192154)

General description

Huntingtin-interacting protein-1 (HIP1), a cytosolic protein, is a member of the huntingtin-interacting protein family. HIP1 and HIP1-related (HIP1R) proteins have the same domain structure, consisting of a phospholipid-binding epsin N-terminal homology (ENTH), a leucine zipper motif, a central coiled-coiled and a C-terminal actin-binding domain (THATCH). The HIP1 gene is expressed broadly in the endothelium of small blood vessels, cardiac muscle, gastric epithelium, brain tissues and liver.

Specificity

Anti-HIP1 (C-terminal) specifically recognizes rat and mouseHIP1.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminus of rat HIP1, conjugated to KLH. The corresponding sequence is highly conserved (89% identity) in mouse HIP1.

Application

Anti-HIP1 (C-terminal) antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

Huntingtin-interacting protein-1 (HIP1) and its homolog HIP1-related (HIP1R) is essential for clathrin-mediated membrane traffic and endocytosis. It is associated with the early recruitment and late-stage maturation of coated pits vesicles. HIP1 is essential for the survival of germline progenitors and several cell lines. It interacts with huntingtin (Htt) and is involved in the pathogenesis of Huntington′s disease (HD). HIP1 binds to clathrin and the endocytic adaptor complex (AP2) and modulates the binding of HIP1 to actin. This leads to the alterations in actin cytoskeleton structure.The fusion between HIP1 and platelet-derived growth factor β receptor might lead to chronic myelomonocytic leukemia. The HIP1 gene is highly expressed in several human cancers, including colon and prostate cancer. It is also present in the breast, melanoma, brain and ovarian tumors. Over-expression of HIP1 transforms fibroblasts and elevates the levels of multiple growth factor receptors.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
HIP1 exhibits an early recruitment and a late stage function in the maturation of coated pits
Gottfried I, et al.
Cellular and Molecular Life Sciences, 66(17), 2897 -22911 (2009)
Huntingtin Interacting Protein 1 mutations lead to abnormal hematopoiesis, spinal defects and cataracts
Oravecz-Wilson KI, et al.
Human Molecular Genetics, 13(8), 851 -8867 (2004)
Huntingtin-interacting protein 1 is overexpressed in prostate and colon cancer and is critical for cellular survival
Rao DS, et al.
The Journal of Clinical Investigation, 110(3), 351 -3360 (2002)
Huntingtin interacting protein 1 Is a clathrin coat binding protein required for differentiation of late spermatogenic progenitors
Rao DS, et al.
Molecular and Cellular Biology, 21(22), 7796 -77806 (2001)
Actin binding by Hip1 (huntingtin-interacting protein 1) and Hip1R (Hip1-related protein) is regulated by clathrin light chain
Wilbur JD, et al.
The Journal of Biological Chemistry, 283(47), 32870 -332879 (2008)

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