SAB4200396

Sigma-Aldrich

Anti-EXOSC2 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):
Anti-HRRP4P, Anti-RRP4P, Anti-Exosome component 2, p7, Anti-RRP4
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~55 kDa

species reactivity

mouse, human, rat

concentration

~1.0 mg/mL

application(s)

western blot: 2.5-5.0 μg/mL using whole extracts of human Jurkat, mouse A20 and rat PC12 cells

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

General description

Exosome component 2 (EXOSC2) gene is mapped to human chromosome 9q34. It comprises S1 RNA-binding domain and is a part of the three-subunit cap and a core exosome component.
EXOSC2 (Exosome component 2) is a non-catalytic subunit of the eukaryotic RNA exosome. It is localized in the nucleus and in the cytoplasm.

Specificity

Anti-EXOSC2 recognizes human, mouse and rat EXOSC2.

Immunogen

peptide corresponding to the N-terminal region of human EXOSC2, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, bovine, pig and dog.

Application

Anti-EXOSC2 antibody produced in rabbit is suitable for western blot at a concentration of 2.5-5.0μg/mL using whole extracts of human Jurkat, mouse A20 and rat PC12 cells.

Biochem/physiol Actions

Exosome component 2 (EXOSC2) gene mutations leads to alterations in its RNA binding and recruiting abilities. Biallelic mutations is implicated in facial gestalt and other associated anomalies.
EXOSC2 (Exosome component 2) is associated with several cellular processes such as rRNA processing, RNA degradation from the 3′ end, 3′ to 5′ decay of the mRNA body after its deadenylation. Exosome complex is an ~400kDa multimeric complex, which plays a vital role in substrate recognition. EXOSC2 functions as a cofactor in the maintenance of human exosome integrity and efficient degradation and/or metabolism of various RNA classes.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage freeze in workingaliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Jungwon Huh et al.
Annals of hematology, 90(11), 1255-1264 (2011-03-09)
The current study investigated molecular cytogenetic characteristics of chronic myeloid leukemia (CML) using genome-wide, single nucleotide polymorphism arrays (SNP-A) capable of detecting cryptic submicroscopic genomic aberrations. Genome-Wide Human SNP 6.0 Array (Affymetrix, CA, USA) was performed in 118 patients having...
Derrick J Morton et al.
RNA (New York, N.Y.), 24(2), 127-142 (2017-11-03)
The RNA exosome is an evolutionarily conserved, ribonuclease complex that is critical for both processing and degradation of a variety of RNAs. Cofactors that associate with the RNA exosome likely dictate substrate specificity for this complex. Recently, mutations in genes...
Nataliya Di Donato et al.
Journal of medical genetics, 53(6), 419-425 (2016-02-05)
Retinitis pigmentosa in combination with hearing loss can be a feature of different Mendelian disorders. We describe a novel syndrome caused by biallelic mutations in the 'exosome component 2' (EXOSC2) gene. Clinical ascertainment of three similar affected patients followed by...
Daniel L Kiss et al.
RNA (New York, N.Y.), 16(4), 781-791 (2010-02-27)
The RNA processing exosome complex was originally defined as an evolutionarily conserved multisubunit complex of ribonucleases responsible for the processing and/or turnover of stable RNAs. The exosome complex is also involved in the surveillance of mRNAs in both the nucleus...
Erwin L van Dijk et al.
RNA (New York, N.Y.), 13(7), 1027-1035 (2007-06-05)
The human exosome is a 3'-5' exoribonuclease complex that functions both in the nucleus and in the cytoplasm to either degrade or process RNA. Little is known yet about potential differences among core exosome complexes in these different cellular compartments...

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