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SAB4200489

Sigma-Aldrich

Anti-Occludin (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

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Synonym(s):
Anti-OCLN
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~65 kDa

species reactivity

human, dog

concentration

~1.0 mg/mL

technique(s)

immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded human colon.
indirect immunofluorescence: 1-2 μg/mL using MDCK cells
western blot: 1.5-3.0 μg/mL using extracts of Caco-2 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

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This Item
SAB4200593SAB5700784SAB4200534
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

species reactivity

human, dog

species reactivity

human

species reactivity

human, mouse, rat

species reactivity

human, dog

mol wt

antigen ~65 kDa

mol wt

-

mol wt

23, 31, 52, 54, 59, 8

mol wt

antigen ~23 kDa

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General description

Occludin (OCLN) is an integral membrane protein, encoded by the gene mapped to human chromosome 5q13.2. OCLN has a molecular weight of about 65kDa. It possesses four transmembrane domains including a long COOH-terminal cytoplasmic domain, a short NH2-terminal cytoplasmic domain, two extracellular loops and one intracellular turn. It is specifically localized at tight junctions (TJ).

Immunogen

a synthetic peptide corresponding to a sequence at the C-terminal region of human occludin, conjugated to KLH. The corresponding sequence is highly conserved in rat (89% sequence identity) and in mouse (84% sequence identity) occludin.

Application

Anti-Occludin (C-terminal) antibody produced in rabbit has been used in immunohistochemistry.

Biochem/physiol Actions

Occludin (OCLN), along with tight junction (TJ)-associated peripheral membrane proteins plays a vital role in the formation and regulation of TJ. The encoded protein facilitates the translocation of p85a (regulatory subunit of phosphoinositide 3-kinase) to TJs to regulate actin organization after oxidative stress. OCLN is also implicated in cell migration. Mutation in the gene leads to the development of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG).

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Recessive Mutations in the Gene Encoding the Tight Junction Protein Occludin Cause Band-like Calcification with Simplified Gyration and Polymicrogyria
Mary C, et al.
American Journal of Human Genetics (2010)
Complex Phenotype of Mice Lacking Occludin, a Component of Tight Junction Strands
Complex Phenotype of Mice Lacking Occludin, a Component of Tight Junction Strands
Mitinouri Saitou, et al
Molecular Biology of the Cell (2017)
Claudin-1 and -2: Novel Integral Membrane Proteins Localizing at Tight Junctions with No Sequence Similarity to Occludin
The Journal of Cell Biology, 141(7) (1998)
The Tight Junction Protein, Occludin, Regulates the Directional Migration of Epithelial Cells
Dan Du, et al.
Developmental Cell (2010)
Splicing Diversity of the Human OCLN Gene and Its Biological Significance for Hepatitis C Virus Entry? ?
Indu Kohaar, et al.
Molecular and Cellular Biology (2010)

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