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SAB4200503

Sigma-Aldrich

Anti-PSPC1 antibody, Mouse monoclonal

enhanced validation

clone 1L4, purified from hybridoma cell culture

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Synonym(s):
Monoclonal Anti-PSP1, Monoclonal Anti-PSPC1 antibody produced in mouse, Monoclonal Anti-paraspeckle component 1
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

1L4, monoclonal

form

buffered aqueous solution

mol wt

antigen ~60 kDa

species reactivity

human, rat, mouse

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 5-10 μg/mL using Human HeLa cells.
western blot: 0.5-1 μg/mL using Whole extracts of mouse Hepa1-6 cells.

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PSPC1(55269)
mouse ... Pspc1(66645)
rat ... Pspc1(305910)

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SAB4200068SAB4200504SAB4200501
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

clone

1L4, monoclonal

clone

polyclonal

clone

SND1-3, monoclonal

clone

FC23, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

antigen ~60 kDa

mol wt

antigen ~59 kDa

mol wt

antigen ~102 kDa

mol wt

antigen ~95 kDa

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General description

Monoclonal Anti-PSPC1 (mouse IgG1 isotype) is derived from the hybridoma 1L4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of mouse PSPC1. DBHS (Drosophila behavior, human splicing) protein family, consisting also of NONO (nonPOU-domain-containing, octamer binding protein) and SFPQ (splicing factor, praline- and glutamine-rich). NONO, paraspeckle component 1 (PSPC1) and SFPQ are the core protein components of paraspeckles. These multi-functional nuclear proteins, under normal conditions, cycle between the nucleoplasm, paraspeckles and the nucleolus.
Paraspeckle component 1 (PSPC1) is encoded by the gene mapped to human chromosome 13q12.11. The encoded protein is a member of DBHS (Drosophila Behavior Human Splicing) family of proteins.

Immunogen

Synthetic peptide corresponding to a sequence at the C-terminal region of mouse PSPC1, conjugated to KLH. 1 The corresponding sequence is identical in rat and human PSPC1.

Application

Monoclonal Anti-PSPC1 antibody produced in mouse has been used in immunohistochemistry, immunoblotting and immunofluorescence.

Biochem/physiol Actions

Drosophila behavior, human splicing (DBHS) proteins bind both double- and single-stranded DNA and RNA, and are involved in various aspects of RNA and DNA metabolism, such as transcription, pre-mRNA 3′ processing, transcription termination and mRNA splicing. DBHS proteins regulate circadian rhythm and carcinogenesis. PSPC1 activates androgen receptor mediated transcription. PSPC1 forms a heterodimer with NONO that localizes to paraspeckles in a RNA polymerase II-dependent manner.
Since paraspeckle component 1 (PSPC1) has lower background nucleoplasmic signal in various cell types compared to other members of DBHS (Drosophila Behavior Human Splicing) family of proteins, it is frequently used as a marker for paraspeckles. The encoded protein plays a vital role in regulation of transcription. Deletion of the gene is associated with the hindered psychomotor development, muscle hypotonia, unilateral microphthalmia with ptosis, congenital eye malformation and facial dysmorphic features.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Paraspeckles: nuclear bodies built on long noncoding RNA
Bond S and Fox AH
The Journal of Cell Biology, 186(5), 637-644 (2009)
Divya Reddy et al.
EMBO reports, 24(1), e55345-e55345 (2022-11-11)
Paraspeckles are subnuclear RNA-protein structures that are implicated in important processes including cellular stress response, differentiation, and cancer progression. However, it is unclear how paraspeckles impart their physiological effect at the molecular level. Through biochemical analyses, we show that paraspeckles
Ana Bajc Česnik et al.
Journal of cell science, 132(5) (2019-02-13)
The GGGGCC (G4C2) repeat expansion mutation in the C9ORF72 gene is the most common genetic cause of frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). Transcription of the repeat and formation of nuclear RNA foci, which sequester specific RNA-binding proteins
Paraspeckles
Fox A H
Cold Spring Harbor Perspectives in Biology, 2 (2010)
Neat1 in hematopoietic stem cells
Fallik N, et al.
Oncotarget, 8(65), 109575-109575 (2017)

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