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SAB4200530

Sigma-Aldrich

Anti-ZNF746 antibody produced in rabbit

enhanced validation

~1.0 mg/mL, affinity isolated antibody

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Synonym(s):
Anti-Parkin-interacting Substrate, PARIS
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~90 kDa

species reactivity

rat, mouse, human

enhanced validation

recombinant expression
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concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 2.5-5.0 μg/mL using SH-SY-5Y cells.
western blot: 1.0-2.0 μg/mL using using rat brain extracts (S2 fraction) and NIH3T3 fibroblast cell extracts.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

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This Item
HPA030090HPA063496HPA042364
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

species reactivity

rat, mouse, human

species reactivity

human

species reactivity

human

species reactivity

human

technique(s)

indirect immunofluorescence: 2.5-5.0 μg/mL using SH-SY-5Y cells., western blot: 1.0-2.0 μg/mL using using rat brain extracts (S2 fraction) and NIH3T3 fibroblast cell extracts.

technique(s)

immunohistochemistry: 1:500- 1:1000

technique(s)

immunofluorescence: 0.25-2 μg/mL

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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General description

Zinc finger protein 746, also known as PARIS, parkin interacting substrate (ZNF746 ) is a 644 amino acid protein. It has a Kruppel-associated box (KRAB) at its N-terminal and a C2HC/C2H2 type zinc finger at its C terminals. This protein is highly conserved among human, mouse and rat, and widely expressed in many tissues.

Immunogen

synthetic peptide corresponding to an internal sequence of human ZNF746, conjugated to KLH. The corresponding sequence is identical in human ZNF746 isoform 2, and in mouse and rat ZNF746.

Application

Anti-ZNF746 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Zinc finger protein 746, also known as PARIS, parkin interacting substrate (ZNF746 ) accumulates in Parkinson′s disease (PD) models and in human PD brain. Hence it play a role in the progress of PD disease. ZNF746 is also regulated by the E3 ubiquitin ligase, parkin. In addition, overexpression of ZNF746 leads to the selective loss of dopaminergic neurons in the substantia nigra.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Zinc finger protein 746 promotes colorectal cancer progression via c-Myc stability mediated by glycogen synthase kinase 3 beta and F-box and WD repeat domain-containing 7
Jung JH, et al.
Oncogene, 1-1 (2018)
Joo-Ho Shin et al.
Cell, 144(5), 689-702 (2011-03-08)
A hallmark of Parkinson's disease (PD) is the preferential loss of substantia nigra dopamine neurons. Here, we identify a new parkin interacting substrate, PARIS (ZNF746), whose levels are regulated by the ubiquitin proteasome system via binding to and ubiquitination by

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