SAB4200530

Anti-ZNF746 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-Parkin-interacting Substrate, PARIS
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IF, WB
• Citations: 2

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~90 kDa
• species reactivity: rat, mouse, human
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 2.5-5.0 μg/mL using SH-SY-5Y cells., western blot: 1.0-2.0 μg/mL using using rat brain extracts (S2 fraction) and NIH3T3 fibroblast cell extracts.
• UniProt accession no.: Q6NUN9
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Quality Level: 200
• Gene Information: human ... ZNF746(155061) ; mouse ... Zfp746(69228) ; rat ... Zfp746(312303)

Description

General description

Zinc finger protein 746, also known as PARIS, parkin interacting substrate (ZNF746 ) is a 644 amino acid protein. It has a Kruppel-associated box (KRAB) at its N-terminal and a C2HC/C2H2 type zinc finger at its C terminals. This protein is highly conserved among human, mouse and rat, and widely expressed in many tissues.

Immunogen

synthetic peptide corresponding to an internal sequence of human ZNF746, conjugated to KLH. The corresponding sequence is identical in human ZNF746 isoform 2, and in mouse and rat ZNF746.

Application

Anti-ZNF746 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Zinc finger protein 746, also known as PARIS, parkin interacting substrate (ZNF746 ) accumulates in Parkinson′s disease (PD) models and in human PD brain. Hence it play a role in the progress of PD disease. ZNF746 is also regulated by the E3 ubiquitin ligase, parkin. In addition, overexpression of ZNF746 leads to the selective loss of dopaminergic neurons in the substantia nigra.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable