SAB4200531

Sigma-Aldrich

Anti-ZNF746 (C-terminal region) antibody produced in rabbit

enhanced validation

~1.0 mg/mL, affinity isolated antibody

Synonym(s):
Anti-Parkin-interacting sustrate, PARIS
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~100 kDa

species reactivity

human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.0 mg/mL

application(s)

immunoprecipitation (IP): 2.0-4.0 μg using lysates of HEK-293T cells overexpressing human ZNF746.
western blot: 0.1-0.2 μg/mL using extracts of HEK-293T cells overexpressing human ZNF746.

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... ZNF746(155061)
mouse ... Zfp746(69228)
rat ... Zfp746(312303)

General description

Zinc finger protein 746 (ZNF746) or PARIS (parkin interacting substrate) contains a kruppel-associated box (KRAB) at its N-terminal and a C2HC/C2H2 type zinc finger at its C-terminal. It belongs to the KRAB-zinc-finger protein (ZFPs) group of transcriptional repressors. ZNF746 is highly conserved among human, mouse and rat, and widely expressed in many tissues. ZNF746 gene is mapped to human chromosome 7q36.1.

Specificity

Anti-ZNF746(C-terminalregion) specifically recognizes human ZNF746.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human ZNF746, conjugated to KLH. The corresponding sequence is identical in human ZNF746 isoform 2, and in mouse and rat ZNF746.

Application

Anti-ZNF746 (C-terminal region) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Zinc finger protein 746 (ZNF746) is regulated by the E3 ubiquitin ligase, parkin. It represses the expression of the transcriptional coactivator, peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α). It also targets nuclear respiratory factor 1 (NRF-1). ZNF746 has been shown to be accumulated in Parkinson′s disease (PD) models and has been suggested to play a role in the progress of PD. Elevated expression of ZNF746 is implicated in the loss of dopamine (DA) neurons in the substantia nigra. This loss of DA neuron is reversed by either coexpression of parkin or PGC-1α. It may serve as a target to inhibit non-small cell lung cancer (NSCLC) invasion.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Tamotsu Nishida et al.
Biochemical and biophysical research communications, 473(4), 1261-1267 (2016-04-19)
Parkin-interacting substrate (PARIS), a member of the family of Krüppel-associated box (KRAB)-containing zinc-finger transcription factors, is a substrate of the ubiquitin E3 ligase parkin. PARIS represses the expression of peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), although the underlying mechanisms remain...
Bonglee Kim et al.
Oncology reports, 31(1), 73-78 (2013-10-23)
Although ZNF746, also known as Parkin-interacting substrate (PARIS), has been reported to suppress peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) and its target gene NRF-1 leading to the neurodegeneration in Parkinson's disease, its function in tumorigenesis has yet to be investigated. Thus...
Joo-Ho Shin et al.
Cell, 144(5), 689-702 (2011-03-08)
A hallmark of Parkinson's disease (PD) is the preferential loss of substantia nigra dopamine neurons. Here, we identify a new parkin interacting substrate, PARIS (ZNF746), whose levels are regulated by the ubiquitin proteasome system via binding to and ubiquitination by...
Mohannad Al Chiblak et al.
BMC molecular and cell biology, 20(1), 60-60 (2019-12-21)
ZNF746 and ZNF777 belong to a subset of the large Krüppel-associated box (KRAB) zinc finger (ZNF) transcription factor family. They contain, like four other members in human, an additional conserved domain, the "domain of unknown function 3669" (DUF3669). Previous work...

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