SAB4200540

Sigma-Aldrich

Anti-HAP1 antibody produced in rabbit

IgG fraction of antiserum

Synonym(s):
Anti-HIP5, Anti-hHLP1, Anti-huntingtin-associated protein 1, Anti-HAP2
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

rabbit

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~70 kDa

species reactivity

human, rat

application(s)

indirect immunofluorescence: 1:100-1:200 using PC-12 cells differentiated with rat NGF.
western blot: 1:1,000-1:2,000 using lysates of A431 cells

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... HAP1(9001)
rat ... 29430(Hap1)

General description

The gene HAP1 (huntingtin-associated protein 1) encodes a nuclear enzyme that is mainly expressed in neurons. It is the human homolog of exonuclease III protein from E. coli. The gene is mapped to human chromosome 17q21.2-q21.3.
Htt-associated protein-1 (HAP1) is present abundantly in the brain. It exists in two isoforms and is located on the human chromosome at 17q21.2.

Specificity

Anti-HAP1 specifically recognizes human and rat HAP1.

Immunogen

synthetic peptide corresponding to an internal sequence of human HAP1, conjugated to KLH. Whole antiserum is purified using protein A immobilized on agarose to provide the IgG fraction of antiserum.

Application

Anti-HAP1 antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

The gene HAP1 (huntingtin-associated protein 1) is a bifunctional enzyme that functions as a DNA repair enzyme as well as in redox activation of transcription factors. It binds to the Huntington′s disease (HD) protein huntingtin in a polyglutamine length-dependent manner. It associates with huntingtin via the cytoskeletal proteins dynactin and pericentriolar autoantigen protein 1 forming coiled-coils. It plays a role in vesicle trafficking by facilitating interactions among cytoskeletal, vesicular and motor proteins. It is an apurinic/apyrimidinic (AP) site-specific DNA repair endonuclease that removes the AP sites produced naturally or by cytotoxic drugs and radiation. It also has RNAse H activity and controls the redox state of certain proto-oncogene products, such as the transcription factor c-Jun. The HAP1 protein is a candidate for pathology associated with HD.
Htt-associated protein-1 (HAP1) binds to the Htt protein due to PolyQ expansion. It plays a role in intracellular trafficking in neurons. HAP1 also functions as an adaptor protein linking vesicles with intracellular transporters. It interacts with dynactin p150Glued and kinesin light chain (KLC). HAP1 takes part in endosomal trafficking and stabilization of internalized epidermal growth factor receptor (EGFR) and γ-aminobutyric acid A receptor (GABAAR). Modulation of HAP1 trafficking is crucial for the stability of tropomyosin-related kinase A (TrkA) and neurite outgrowth. HAP1 is also involved in the interaction with pro-brain-derived neurotrophic factor (pro-BDNF) and mediates its transport and release. HAP-1 is associated with pathogenesis of Huntington′s disease.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
HAP1 and intracellular trafficking
Li X J, et al.
Trends in Pharmacological Sciences, 26(1), 1-3 (2005)
Jing Wu et al.
Biochemical and biophysical research communications, 456(1), 162-166 (2014-12-03)
The purpose of this study was to investigate the relationship between huntingtin-associated protein1 (HAP1) gene and radiation therapy of breast cancer cells. HAP1 gene was transfected into breast cancer MCF-7 cells, which was confirmed by quantitative reverse transcription-polymerase chain reaction...
Linda Lin-yan Wu et al.
The Journal of biological chemistry, 285(8), 5614-5623 (2009-12-10)
Brain-derived neurotrophic factor (BDNF) plays a pivotal role in brain development and synaptic plasticity. It is synthesized as a precursor (pro-BDNF), sorted into the secretory pathway, transported along dendrites and axons, and released in an activity-dependent manner. Mutant Huntingtin with...
Interaction of Huntingtin-associated Protein-1 with Kinesin Light Chain IMPLICATIONS IN INTRACELLULAR TRAFFICKING IN NEURONS
McGuire J R, et al.
The Journal of Biological Chemistry, 281(6), 3552-3559 (2006)
Xiao-Jiang Li et al.
Trends in pharmacological sciences, 26(1), 1-3 (2005-01-05)
Huntington's disease is caused by a polyglutamine expansion in the protein huntingtin. Several studies suggest that huntingtin and its associated protein HAP1 participate in intracellular trafficking and that polyglutamine expansion affects vesicular transport. A study now provides new evidence that...

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