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SAB4200544

Sigma-Aldrich

Anti-Synaptophysin antibody, Mouse monoclonal

clone SVP-38, purified from hybridoma cell culture

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Synonym(s):
Monoclonal Anti-MRXSYP, Monoclonal Anti-SYP, Monoclonal Anti-Synaptophysin1, Monoclonal Anti-Synaptoporin, Monoclonal Anti-Syp1, Monoclonal Anti-p38
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

SVP-38, monoclonal

form

buffered aqueous solution

mol wt

antigen 38 kDa

species reactivity

rat, human

concentration

~1.0 mg/mL

technique(s)

immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded rat cerebellum.
indirect immunofluorescence: 10-20 μg/mL using B35 cells.
western blot: 1-2 μg/mL using newborn rat brain extracts.

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SYP(6855)

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conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

clone

SVP-38, monoclonal

clone

SVP-38, monoclonal

clone

1L4, monoclonal

clone

PIWIL1-2, monoclonal

form

buffered aqueous solution

form

-

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

antigen 38 kDa

mol wt

antigen 38 kDa

mol wt

antigen ~60 kDa

mol wt

antigen ~95 kDa

General description

Anti-Synaptophysin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma SVP-38 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synaptosome preparation from rat retina. Synaptophysin is a glycoprotein containing four transmembrane domains with both N and C termini on the cytoplasm face.
Synaptophysin (SYP) is encoded by the gene mapped to human chromosome Xp11.23-p11.22. The encoded protein has a molecular mass of 38kDa and is exclusively localized to the membrane of presynaptic vesicles.

Specificity

Anti-Synaptophysin antibody, Mouse monoclonal recognizes rat, human, guinea-pig, and pig synaptophysin

Immunogen

synaptosome preparation from rat retina. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor.

Application

Anti-Synaptophysin antibody, Mouse monoclonal has been used in following immunochemical techniques:
  • Immunoblotting
  • Immunohistochemistry
  • Immunofluorescence

Biochem/physiol Actions

Synaptophysin (SYP) facilitates neurotransmitter release and synaptic plasticity. It is also implicated in synaptic vesicle formation and exocytosis. Mutation in the gene increases the risk of susceptibility to schizophrenia. It is widely used as a potential marker to study the circulation of synapses in the brain and to discover the basic features of the life cycle of synaptic vesicles (SVs).
Synaptophysin has also been implicated in the recycling of synaptic vesicles by associating with dynamin I, a GTPase required for endocytosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles
Wiedenmann B and Franke WW
Cell, 41, 1017-1028 (1985)
Synaptophysin: leading actor or walk-on role in synaptic vesicle exocytosis?
Valtorta F
Bioessays, 26, 445-453 (2004)
Differential susceptibility of striatal, hippocampal and cortical neurons to Caspase-6
Noel A, et al.
Cell Death and Differentiation, 25(7), 1319-1319 (2018)
Neuronal connections of direct and indirect pathways for stable value memory in caudal basal ganglia
Amita H, et al.
The European Journal of Neuroscience, 49(5), 712-725 (2019)
Sequence-based exon prediction around the synaptophysin locus reveals a gene-rich area containing novel genes in human proximal Xp.
Fisher SE
Genomics, 45, 340-347 (1997)

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