MilliporeSigma
All Photos(4)

Documents

SAB4200646

Sigma-Aldrich

Monoclonal Anti-ALIX/PDCD6IP antibody produced in mouse

clone ALIX-1, purified from hybridoma cell culture

Sign Into View Organizational & Contract Pricing

NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ALIX-1, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

~1 mg/mL

technique(s)

flow cytometry: 5-10 μg/test using using human MOLT4 cells
immunoblotting: 2-5 μg/mL using using whole extracts of human MOLT4 cells.
immunofluorescence: 10-20 μg/mL using using human HeLa cells
immunoprecipitation (IP): 10-20 μg/mL using using lysates of human MOLT4 cells.

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PDCD6IP(10015)

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SAB4200477SAB4200476SAB4200650
clone

ALIX-1, monoclonal

clone

polyclonal

clone

polyclonal

clone

Ep2.17, monoclonal

species reactivity

human

species reactivity

human, mouse

species reactivity

mouse, human

species reactivity

human

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

Gene Information

human ... PDCD6IP(10015)

Gene Information

human ... PDCD6IP(10015)
mouse ... Pdcd6ip(18571)

Gene Information

human ... PDCD6IP(10015)
mouse ... Pdcd6ip(18571)

Gene Information

human ... SH3GL1(6455)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Anti-ALIX/PDCD6IP antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma ALIX-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to an internal region of human ALIX/ PDCD6IP, conjugated to KLH. ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X), also named Programmed Cell Death 6 interacting protein (PDCD6IP).

Immunogen

peptide corresponding to an internal region of human ALIX/ PDCD6IP

Application

Monoclonal Anti-ALIX/PDCD6IP antibody has been used in
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence
  • flow cytometry

Biochem/physiol Actions

ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X) plays a role in ESCRT (endosomal sorting complexes required for transport) pathway. It is involved in the abscission stage of cytokinesis, in intralumenal endosomal localization, and in enveloped virus budding. Overexpression of this protein can block apoptosis. In addition, ALIX binds to PDCD6, a protein required for apoptosis, in a calcium-dependent manner. ALIX also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of ALIX and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

The multiple personalities of Alix
Odorizzi G.
Journal of Cell Science, 119(15), 3025-3032 (2006)
AIP1/Alix is a binding partner of Sendai virus C protein and facilitates virus budding
Sakaguchi T, et al.
Journal of Virology, 79(14), 8933-8941 (2005)
Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis
Morita E, et al.
The Embo Journal, 26(19), 4215-4227 (2007)
Xuefeng Ren et al.
Traffic (Copenhagen, Denmark), 12(10), 1282-1290 (2011-04-27)
Most membrane-enveloped viruses bud from infected cells by hijacking the host ESCRT machinery. The ESCRTs are recruited to the budding sites by viral proteins that contain short proline (Pro)-rich motifs (PRMs) known as late domains. The late domains probably evolved
Christine Chatellard-Causse et al.
The Journal of biological chemistry, 277(32), 29108-29115 (2002-05-30)
ALG-2-interacting protein X (Alix), also known as AIP1, is a cytoplasmic protein ubiquitously expressed and concentrated in phagosomes and exosomes. Alix may regulate apoptosis since it binds apoptosis-linked gene 2 (ALG-2), a Ca2+-binding protein necessary for cell death, and also

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service