SAB4200655

Sigma-Aldrich

Monoclonal Anti-NCAPH2 antibody produced in rat

clone 5F2G4, purified from hybridoma cell culture

NACRES:
NA.41

Quality Level

biological source

rat

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5F2G4, monoclonal

form

buffered aqueous solution

species reactivity

human, rat, mouse

concentration

~1 mg/mL

application(s)

flow cytometry: 1-2 μg/test using using HeLa cells
immunoblotting: 2-4 μg/mL using using whole extracts of HeLa cells
immunofluorescence: 0.25-0.5 μg/mL using using HeLa cells

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... NCAPH2(29781)
mouse ... Ncaph2(52683)
rat ... Ncaph2(300149)

General description

Non-SMC condensin II complex subunit H2 (NCAPH2) is one of the subunits of the condensin II complex. The gene encoding it is localized on human chromosome 22q13.

Immunogen

a human NCAPH2 (full-length) recombinant fusion protein

Application

Monoclonal Anti-NCAPH2 antibody produced in rat has been used in immunostaining.

Biochem/physiol Actions

Non-SMC condensin II complex subunit H2 (NCAPH2) binds to mitotic chromosomes and is associated with mitosis. Mutations in the gene encoding NCAPH2 have been shown to impair T-cell functions in mice. The NCAPH2 gene is associated with mitosis and meiosis, which predicts that its methylation influences apoptosis of neural cells. NCAPH2 methylation causes loss of hippocampal function and cognitive dysfunction leading to progressive dementia.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Dynamic Organization of Chromatin Domains Revealed by Super-Resolution Live-Cell Imaging
Tadasu N, et al.
Molecular Cell, 67(2), 282-293 (2017)
Katharine M Gosling et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(30), 12445-12450 (2007-07-21)
Condensins are ubiquitously expressed multiprotein complexes that are important for chromosome condensation and epigenetic regulation of gene transcription, but whose specific roles in vertebrates are poorly understood. We describe a mouse strain, nessy, isolated during an ethylnitrosourea screen for recessive...
Condensin II and GAIT complexes cooperate to restrict LINE-1 retrotransposition in epithelial cells.
Jacqueline R W, et al.
PLoS ONE, 13(10), e1007051-e1007051 (2017)
Takao Ono et al.
Cell, 115(1), 109-121 (2003-10-09)
The canonical condensin complex (henceforth condensin I) plays an essential role in mitotic chromosome assembly and segregation from yeast to humans. We report here the identification of a second condensin complex (condensin II) from vertebrate cells. Condensins I and II...
A novel role for the condensin II complex in cellular senescence.
Yokoyama Y
Cell Cycle, 14(13), 2160-2170 (2015)

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