This product is not explicitly validated for immunohistochemistry on frozen sections. It is validated for immunohistochemistry on paraffin-embedded sections at 2.5–5 µg/mL and for Western blotting at 1.25–2.5 µg/mL. However, antibodies from the same clone, NOQ7.5.4D, have been reported to work in immunohistochemistry on frozen sections and also cross-react with human slow skeletal myosin. This product is expected to be suitable for human skeletal muscle cryosections, but in-house optimization is recommended.
SAB4200670
Anti-Myosin (Skeletal, Slow) antibody, Mouse monoclonal
clone NOQ7.5.4D, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-Myosin (Skeletal, Slow) antibody produced in mouse, CMD1S, CMH1, MPD1, MYHCB, SPMD, SPMM
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
NOQ7.5.4D, monoclonal
Species reactivity:
sheep, goat, chicken, feline, guinea pig, pig, rabbit, hamster, canine, rat, human, mouse, bovine
Application:
IHC
Citations:
8
Pricing and availability is not currently available.
biological source
mouse
Quality Segment
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
NOQ7.5.4D, monoclonal
form
buffered aqueous solution
species reactivity
sheep, goat, chicken, feline, guinea pig, pig, rabbit, hamster, canine, rat, human, mouse, bovine
packaging
antibody small pack of 25 μL
concentration
~1 mg/mL
technique(s)
immunoblotting: 1.25-2.5 μg/mL using Rat skeletal muscle lysate., immunohistochemistry: 2.5-5 μg/mL using pronase digested, formalin-fixed, paraffin-embedded rabbit tongue/leg sections and Biotin/ExtrAvidin®-Peroxidase staining system.
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MYH7(4625)
General description
Monoclonal Anti-Myosin (Skeletal, Slow) (mouse IgG1 isotype) is derived from the NOQ7.5.4D hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Myosin is a hexameric protein containing two heavy chain subunits and four light chain subunits. It is a major protein component of heart and skeletal muscles. Muscle myosin heavy chain (MHC) different isoforms are expressed depending on the physiological function of the muscle: skeletal, cardiac, smooth and non-muscle.
Immunogen
Myosin, purified from myofibrils isolated from human skeletal muscle,
Application
Monoclonal Anti-Myosin (Skeletal, Slow) antibody produced in mouse has been used in immunobloting, immunohistology, Solid-phase radioimmunoassay (RIA), enzyme linked immunosorbent assay (ELISA) and immunoelectronmicroscopy.
Biochem/physiol Actions
Myosin are actin-dependent motors. Mutations in myosins genes are associated with myopathies, blindness and hearing loss. They are involved in muscle contraction, vision, hearing, cell movements and cell invasion.
Myosin is the element responsible for energy transduction and force development in heart and skeletal muscles. Myosin is known to interact with actin in muscle and in nonmuscle cells. Slow myosin, also known as β-myosin heavy chain (β-MHC) is expressed during embryogenesis as part of the cardiac myogenic program under the control of NK-2 homeobox protein-5 (NKX-2.5), myocyte enhancer factor-2C (MEF-2C), and GATA-4/5/6.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Legal Information
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| antibody form purified from hybridoma cell culture | antibody form ascites fluid | antibody form ascites fluid | antibody form whole antiserum |
| species reactivity sheep, goat, chicken, feline, guinea pig, pig, rabbit, hamster, canine, rat, human, mouse, bovine | species reactivity sheep, rat, bovine, hamster, pig, canine, feline, goat, chicken, mouse, rabbit, human, guinea pig | species reactivity rat, chicken, rabbit, mouse, human, bovine, guinea pig, feline | species reactivity human |
| biological source mouse | biological source mouse | biological source mouse | biological source rabbit |
| Gene Information human ... MYH7(4625) | Gene Information human ... MYH7(4625) | Gene Information human ... MYH1(4619), MYH2(4620) | Gene Information human ... MYH13(8735), MYH3(4621), MYL1(4632) |
| clone NOQ7.5.4D, monoclonal | clone NOQ7.5.4D, monoclonal | clone MY-32, monoclonal | clone polyclonal |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Is the antibody SAB4200670 reactive on human skeletal muscle frozen sections (non-paraffin fixed) in immunohistochemistry?
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