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SAB4200670

Sigma-Aldrich

Anti-Myosin (Skeletal, Slow) antibody, Mouse monoclonal

clone NOQ7.5.4D, purified from hybridoma cell culture

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Synonym(s):
Monoclonal Anti-Myosin (Skeletal, Slow) antibody produced in mouse, CMD1S, CMH1, MPD1, MYHCB, SPMD, SPMM
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

NOQ7.5.4D, monoclonal

form

buffered aqueous solution

species reactivity

sheep, goat, chicken, feline, guinea pig, pig, rabbit, hamster, canine, rat, human, mouse, bovine

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunoblotting: 1.25-2.5 μg/mL using Rat skeletal muscle lysate.
immunohistochemistry: 2.5-5 μg/mL using pronase digested, formalin-fixed, paraffin-embedded rabbit tongue/leg sections and Biotin/ExtrAvidin®-Peroxidase staining system.

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MYH7(4625)

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This Item
M8421M4276M1570
species reactivity

sheep, goat, chicken, feline, guinea pig, pig, rabbit, hamster, canine, rat, human, mouse, bovine

species reactivity

sheep, rat, bovine, hamster, pig, canine, feline, goat, chicken, mouse, rabbit, human, guinea pig

species reactivity

rat, chicken, rabbit, mouse, human, bovine, guinea pig, feline

species reactivity

rat, chicken, rabbit, mouse, human, bovine, guinea pig, feline

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

Gene Information

human ... MYH7(4625)

Gene Information

human ... MYH7(4625)
mouse ... Myh7(140781)
rat ... Myh7(29557)

Gene Information

human ... MYH1(4619), MYH2(4620)
mouse ... Myh1(17879), Myh2(17882)
rat ... Myh1(287408), Myh2(691644)

Gene Information

human ... MYH1(4619), MYH2(4620)
mouse ... Myh1(17879), Myh2(17882)
rat ... Myh1(287408), Myh2(691644)

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

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General description

Monoclonal Anti-Myosin (Skeletal, Slow) (mouse IgG1 isotype) is derived from the NOQ7.5.4D hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Myosin is a hexameric protein containing two heavy chain subunits and four light chain subunits. It is a major protein component of heart and skeletal muscles. Muscle myosin heavy chain (MHC) different isoforms are expressed depending on the physiological function of the muscle: skeletal, cardiac, smooth and non-muscle.

Immunogen

Myosin, purified from myofibrils isolated from human skeletal muscle,

Application

Monoclonal Anti-Myosin (Skeletal, Slow) antibody produced in mouse has been used in immunobloting, immunohistology, Solid-phase radioimmunoassay (RIA), enzyme linked immunosorbent assay (ELISA) and immunoelectronmicroscopy.

Biochem/physiol Actions

Myosin are actin-dependent motors. Mutations in myosins genes are associated with myopathies, blindness and hearing loss. They are involved in muscle contraction, vision, hearing, cell movements and cell invasion.
Myosin is the element responsible for energy transduction and force development in heart and skeletal muscles. Myosin is known to interact with actin in muscle and in nonmuscle cells. Slow myosin, also known as β-myosin heavy chain (β-MHC) is expressed during embryogenesis as part of the cardiac myogenic program under the control of NK-2 homeobox protein-5 (NKX-2.5), myocyte enhancer factor-2C (MEF-2C), and GATA-4/5/6.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Heavy and light roles: myosin in the morphogenesis of the heart
England J and Loughna S
Cellular and Molecular Life Sciences, 70(7), 1221-1239 (2013)
Bernardo J Foth et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(10), 3681-3686 (2006-03-01)
Myosins are eukaryotic actin-dependent molecular motors important for a broad range of functions like muscle contraction, vision, hearing, cell motility, and host cell invasion of apicomplexan parasites. Myosin heavy chains consist of distinct head, neck, and tail domains and have
Jelle C B C de Jong et al.
Biology of sex differences, 14(1), 45-45 (2023-07-11)
Physical weakness is a key component of frailty, and is highly prevalent in older adults. While females have a higher prevalence and earlier onset, sex differences in the development of frailty-related physical weakness are hardly studied. Therefore, we investigated the
Caloric Restriction Combined with Immobilization as Translational Model for Sarcopenia Expressing Key-Pathways of Human Pathology.
de Jong, et al.
Aging and disease, 14, 937-957 (2023)
Yuta Ozaki et al.
Nature communications, 14(1), 4675-4675 (2023-08-05)
To maintain and restore skeletal muscle mass and function is essential for healthy aging. We have found that myonectin acts as a cardioprotective myokine. Here, we investigate the effect of myonectin on skeletal muscle atrophy in various male mouse models

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