SAB4200689

Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal

clone 5C5, hybridoma cell culture supernatant

• Synonym(s): Actin alpha sarcomeric/skeletal, a-SCA, alpha-SCA, alpha-sarcomeric actin
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: 5C5, monoclonal
• Species reactivity: rat, chicken, bovine, mouse, guinea pig, rabbit, human, planaria(flatworm), fish, Xenopus
• Application: ELISA, IF, IHC
• Citations: 7

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5C5, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~42 kDa
• species reactivity: rat, chicken, bovine, mouse, guinea pig, rabbit, human, planaria(flatworm), fish, Xenopus
• packaging: antibody small pack of 25 μL
• technique(s): ELISA: suitable, immunoblotting: 1:500-1:1000 using rat heart tissue extracts, immunofluorescence: 1:500-1000 using human HeLa cells., immunohistochemistry: 1:500 using formalin-fixed, paraffin-embedded human tongue sections and Biotin/ExtrAvidin^®-Peroxidase staining system.
• isotype: IgM
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ACTA2(59)

Description

General description

Monoclonal Anti-Actin (-Sarcomeric) (mouse IgM isotype) is derived from the hybridoma 5C5 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with purified rabbit striated muscle. Actin is the major cytoskeletal protein in eukaryotic cells.

Immunogen

purified rabbit striated muscle

Application

Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal has been used in:

• indirect immunofluorescence staining
• immunohistochemistry
• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunofluorescence

Biochem/physiol Actions

Actin plays essential roles in a number of cellular processes including cell migration, cytokinesis, vesicle transport and contractile force generation.

Physical form

The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains bovine serum albumin and a human-derived protein.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable