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SAB4200690

Sigma-Aldrich

Anti-EpCAM antibody, Mouse monoclonal

clone Ber-EP4, hybridoma cell culture supernatant

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Synonym(s):
Anti-CD326 antigen, Anti-EGP, Anti-Epithelial cell surface antigen, Anti-Epithelial glycoprotein, Anti-KS 1/4 antigen, Anti-KSA, Anti-Major gastrointestinal tumor-associated protein GA733-2, Anti-Tumor-associated calcium signal transducer 1

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

Ber-EP4, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

immunofluorescence: 1:500-1:1,000 using human breast adenocarcinoma MCF-7 cell line.
immunohistochemistry: 1:250-1:500 using heat-retrieved formalin-fixed, paraffin-embedded human colon carcinoma sections.
immunoprecipitation (IP): suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EPCAM(4072)

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This Item
SAB4200473MABT1587SAB4700423
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

Gene Information

human ... EPCAM(4072)

Gene Information

human ... EPCAM(4072)
mouse ... Epcam(17075)

Gene Information

human ... EPCAM(4072)

Gene Information

human ... EPCAM(4072)

antibody form

culture supernatant

antibody form

purified from hybridoma cell culture

antibody form

purified antibody

antibody form

purified immunoglobulin

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

clone

Ber-EP4, monoclonal

clone

4A7, monoclonal

clone

EpMab?16, monoclonal

clone

VU-1D9, monoclonal

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General description

Anti-EpCAM antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma Ber-EP4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human breast carcinoma cell line MCF-7. EpCAM (Epithelial cell adhesion molecule) is a highly conserved type I transmembrane glycoprotein. EpCAM is present on most epithelia tissues of the adult body and in undifferentiated rather than differentiated embryonic stem cells.

Immunogen

human breast carcinoma cell line MCF-7

Application

Anti-EpCAM antibody has been used in:
  • immunofluorescence
  • immunohistochemistry
  • immunoprecipitation

Biochem/physiol Actions

EpCAM (Epithelial cell adhesion molecule) regulates cell-cell contact adhesion strength and tissue plasticity and epithelial cell proliferation and differentiation. Mutations in EpCAM are associated with several intestinal abnormalities such as Lynch syndrome, and congenital tufting enteropathy (CTE). Anti-EpCAM has been proved valuable for the distinction of undifferentiated primary or metastatic tumors from non-epithelial tumors, bile duct cells from hepatocytes in certain liver diseases, and between epithelial and normal reactive or neoplastic mesothelial cells from carcinoma cells.

Physical form

The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains bovine serum albumin and a human-derived protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Risk of colorectal and endometrial cancers in EPCAM deletion-positive Lynch syndrome: a cohort study
Kempers MJE, et al.
Lancet Oncology, 12(1), 49-55 (2011)
Epithelial cell adhesion molecule: more than a carcinoma marker and adhesion molecule
Trzpis M, et al.
The American Journal of Pathology, 171(2), 386-395 (2007)
The emerging role of EpCAM in cancer and stem cell signaling
Munz M, et al.
Cancer Research, 69(14), 5627-5629 (2009)
Identification of EpCAM as the gene for congenital tufting enteropathy
Sivagnanam M, et al.
Gastroenterology, 135(2), 429-437 (2008)
Ming Liu et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(11), 6103-6113 (2020-03-04)
Clinical observation of the association between cancer aggressiveness and embryonic development stage implies the importance of developmental signals in cancer initiation and therapeutic resistance. However, the dynamic gene expression during organogenesis and the master oncofetal drivers are still unclear, which

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