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SAB4200704

Sigma-Aldrich

Anti-EpCAM antibody, Mouse monoclonal

clone Ber-EP4, purified from hybridoma cell culture

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Synonym(s):
Anti-CD326 antigen, Anti-EGP, Anti-Epithelial cell surface antigen, Anti-Epithelial glycoprotein, Anti-KS 1/4 antigen, Anti-KSA, Anti-Major gastrointestinal tumor-associated protein GA733-2, Anti-Tumor-associated calcium signal transducer 1
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

Ber-EP4, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

~1 mg/mL

technique(s)

immunofluorescence: 1-2 μg/mL using human breast adenocarcinoma MCF-7 cell line.
immunohistochemistry: 5-10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human colon carcinoma sections.
immunoprecipitation (IP): suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EPCAM(4072)

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SAB4200690SAB4200473SAB4700423
antibody form

purified immunoglobulin

antibody form

culture supernatant

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

clone

Ber-EP4, monoclonal

clone

Ber-EP4, monoclonal

clone

4A7, monoclonal

clone

VU-1D9, monoclonal

Gene Information

human ... EPCAM(4072)

Gene Information

human ... EPCAM(4072)

Gene Information

human ... EPCAM(4072)
mouse ... Epcam(17075)

Gene Information

human ... EPCAM(4072)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

technique(s)

immunofluorescence: 1-2 μg/mL using human breast adenocarcinoma MCF-7 cell line., immunoprecipitation (IP): suitable, immunohistochemistry: 5-10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human colon carcinoma sections.

technique(s)

immunofluorescence: 1:500-1:1,000 using human breast adenocarcinoma MCF-7 cell line. , immunohistochemistry: 1:250-1:500 using heat-retrieved formalin-fixed, paraffin-embedded human colon carcinoma sections., immunoprecipitation (IP): suitable

technique(s)

indirect immunofluorescence: 5-10 μg/mL using human MCF7 cells., western blot: 0.25-0.5 μg/mL using whole extracts of human SW480 cells.

technique(s)

western blot: suitable

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General description

The epithelial cell adhesion molecule (EpCAM) is mapped to human chromosome 2p21 and has nine exons. EpCAM is a 40 kDa transmembrane glycoprotein present on cell surface. It has an intracellular domain called the EpICD, comprising of 26-amino acids. The extracellular domain (EpEX) has an epidermal growth factor (EGF) and a thyroglobulin repeat-like domain.

Immunogen

human breast carcinoma cell line MCF-7

Biochem/physiol Actions

The epithelial cell adhesion molecule (EpCAM) is highly expressed in carcinomas. It regulates cell signalling, proliferation, migration and adhesion. Proteolytic cleavage of EpICD domain is crucial for tumor progression. EpCAM is highly expressed in breast cancer and is considered as a potential target for gene therapy. Deletions in the 3′ region of the EpCAM gene is associated with colorectal cancer based lynch syndrome. Single nucleotide polymorphism in EpCAM gene is associated with infant diarrheal disorder, congenital tufting enteropathy(CTE).

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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EpCAM is overexpressed in breast cancer and is a potential target for breast cancer gene therapy
Osta WA, et al.
Cancer Research, 64(16), 5818-5824 (2004)
Heritable somatic methylation and inactivation of MSH2 in families with Lynch syndrome due to deletion of the 3? exons of TACSTD1
Ligtenberg MJL, et al.
Nature Genetics, 41(1), 112-112 (2009)
EpCAM in carcinogenesis: the good, the bad or the ugly
van der Gun BT, et al.
Carcinogenesis, 31(11), 1913-1921 (2010)
Nadim Aizarani et al.
Nature, 572(7768), 199-204 (2019-07-12)
The human liver is an essential multifunctional organ. The incidence of liver diseases is rising and there are limited treatment options. However, the cellular composition of the liver remains poorly understood. Here we performed single-cell RNA sequencing of about 10,000
EpCAM: structure and function in health and disease
Schnell U, et al.
Biochimica et Biophysica Acta - Biomembranes, 1828(8), 1989-2001 (2013)

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