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SAB4200709

Sigma-Aldrich

Anti-Collagen Type IV antibody, Mouse monoclonal

clone col-94, purified from hybridoma cell culture

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Synonym(s):
Anti-col4a1/col4a2
NACRES:
NA.41

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

col-94, monoclonal

form

buffered aqueous solution

species reactivity

monkey, human

concentration

~1 mg/mL

technique(s)

dot blot: suitable
electron microscopy: suitable
immunohistochemistry: 4-8 μg/mL using formalin-fixed, paraffin-embedded human tongue sections

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... COL4A1(1282)

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This Item
C1926MAB3326SAB4200749
clone

col-94, monoclonal

clone

COL-94, monoclonal

clone

IV-4H12, monoclonal

clone

FH-7A, monoclonal

form

buffered aqueous solution

form

-

form

-

form

buffered aqueous solution

species reactivity

monkey, human

species reactivity

human

species reactivity

human

species reactivity

rat, human

concentration

~1 mg/mL

concentration

-

concentration

-

concentration

~1.0 mg/mL

technique(s)

dot blot: suitable, electron microscopy: suitable, immunohistochemistry: 4-8 μg/mL using formalin-fixed, paraffin-embedded human tongue sections

technique(s)

dot blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested sections of human tissue, immunohistochemistry (frozen sections): suitable, indirect ELISA: suitable, microarray: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

ELISA: suitable, immunoblotting: suitable, immunofluorescence: suitable, immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded rat skin sections.

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General description

Anti-Collagen Type IV antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the col-94 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with human collagen Type IV. Collagen Type IV (also known as COL4A) is a key component of the basement membranes (BM), which is found at the basal surface of epithelial and endothelial cells and is essential for tissue strength and polarity. Type IV Collagens are encoded by three pairs of paralogous genes (COL4A1 through COL4A6).

Immunogen

human collagen Type IV

Application

Anti-Collagen Type IV antibody, Mouse monoclonal has been used in immunohistochemistry and dot-blotting.

Biochem/physiol Actions

Collagen Type IV represents a physical barrier against cell invasion. Proteolysis/degradation of Collagen Type IV is essential for cell migration and cancer cells invasion through the ECM into the interstitial tissues.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Dynamic interplay between the collagen scaffold and tumor evolution
Egeblad M, et al.
Current Opinion in Cell Biology, 22(5), 697-706 (2010)
Sarah N Mapelli et al.
Cancers, 12(1) (2020-01-16)
In this study, we extracted prostate cell-specific gene sets (metagenes) to define the epithelial differentiation status of prostate cancers and, using a deconvolution-based strategy, interrogated thousands of primary and metastatic tumors in public gene profiling datasets. We identified a subgroup
Debbie S Kuo et al.
Human molecular genetics, 21(R1), R97-110 (2012-08-24)
Heterotrimers composed of collagen type IV alpha 1 (COL4A1) and alpha 2 (COL4A2) constitute one of the most abundant components of nearly all basement membranes. Accordingly, mutations in COL4A1 or COL4A2 are pleiotropic and contribute to a broad spectrum of

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