SAB4200713

Sigma-Aldrich

Anti-Human IgG (γ-chain specific) antibody, Mouse monoclonal

clone GG-5, purified from hybridoma cell culture

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

GG-5, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

~1 mg/mL

application(s)

indirect ELISA: 0.25-0.5 μg/mL using 5μg/mL human IgG for coating

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

General description

Anti-Human IgG (γ-chain specific) antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the GG-5 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with purified human IgG. Immunoglobulins (Igs) are produced by B lymphocytes and secreted into the plasma. Immunoglobulins can be divided into five main classes/isotypes which are IgA, IgD, IgE, IgG, and IgM. IgG class identity is determined by class-specific sequences in the Fc region of the heavy chain. IgG antibody class are the most abundant immunoglobulins isotype in blood, lymph fluid, cerebrospinal fluid and peritoneal fluid. IgGs include four subclasses (IgG1, IgG2, IgG3, and IgG4). The IgG subclasses differ in their physical and chemical properties. Their distribution pattern is found to be age dependent and every subclass has a specific biological function.

Immunogen

purified human IgG

Application

Anti-Human IgG (γ-chain specific) antibody, Mouse monoclonal has been used in indirect ELISA.

Biochem/physiol Actions

Immunoglobulins (Igs) serve as key players in the humoral immune response.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Patricia Colque-Navarro et al.
Clinical and vaccine immunology : CVI, 17(7), 1117-1123 (2010-05-07)
Serum samples from 151 healthy individuals aged from 15 to 89 years were investigated by enzyme-linked immunosorbent assay (ELISA) for IgG levels against 11 different purified antigens from Staphylococcus aureus. Surface antigens, such as teichoic acid, clumping factors A and...
Maha Zohra Ladjemi et al.
The European respiratory journal, 45(4), 980-993 (2014-12-30)
Despite their relevance to mucosal defense, production of IgA and the function of lung B-cells remain unknown in chronic obstructive pulmonary disease (COPD). We assessed IgA synthesis in the lungs of COPD (n=28) and control (n=21) patients, and regulation of...
R Jefferis et al.
Annales de biologie clinique, 52(1), 57-65 (1994-01-01)
Secondary systemic immune responses are predominantly of the IgG class and passive administration of intravenous IgG, from pooled normal serum, is an effective prophylactic and/or therapeutic treatment for patients with defined immunodeficiencies. However, the proportions of each IgG subclass present...
N Ruijne et al.
Clinical and vaccine immunology : CVI, 13(7), 797-801 (2006-07-11)
Vaccine trials with infants enrolled between 6 and 10 weeks of age (young infants) and 6 and 8 months of age (older infants) provided an opportunity to evaluate immunoglobulin G (IgG) isotype distribution and avidity maturation as indicators of antibody...
C B Reimer et al.
Hybridoma, 3(3), 263-275 (1984-01-01)
Stable clones of 31 mouse hybridomas that produce monoclonal antibodies (MAbs) against human IgG antigenic determinants were obtained. The number of hybridomas of different specificity described are: 2 anti-IgG1 Fc, 1 anti-IgG2 Fc, 1 anti-IgG2 Fd, 2 anti-IgG3 Fc, 2...

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