SAB4200716

Sigma-Aldrich

Anti-Vimentin antibody, Mouse monoclonal

clone VIM-13.2, purified from hybridoma cell culture

NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

VIM-13.2, monoclonal

form

buffered aqueous solution

mol wt

~58 kDa

species reactivity

rat, chicken, monkey, human, bovine, canine, mouse

concentration

~1.0 mg/mL

application(s)

immunoblotting: 0.25-0.5 μg/mL using human foreskin fibroblast HS68 cell line extract
immunofluorescence: 1.25-2.5 μg/mL using human foreskin fibroblast HS68 cells
immunohistochemistry: suitable

isotype

IgM

shipped in

dry ice

storage temp.

−20°C

General description

Anti-Vimentin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the VIM-13.2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with human foreskin fibroblast extract. Vimentin is a type III intermediate filaments (IF) expressed in a wide range of cell types. including, pancreatic precursor cells, fibroblasts and sertoli cells. It is also expressed in trophoblastic giant cells, neuronal precursor cells, lung epithelial cells, renal tubular and stromal cells. Vimentin is also present in leucocytes, macrophages, neutrophils and mesangial cells.

Immunogen

human foreskin fibroblast extract

Application

Anti-Vimentin antibody, Mouse monoclonal has been used in:
  • western blotting
  • indirect immunofluorescence
  • immunohistochemistry

Biochem/physiol Actions

Vimentin plays a significant role in supporting and anchoring the cellular position of organelles, such as nucleus, endoplasmic reticulum, mitochondria (either laterally or terminally). It helps to maintain the mesenchymal cells especially their shape, motility, survival during of mechanical stress. Epithelial cancers like prostate, breast, lung show an increased vimentin expression. Vimentin is also highly expressed in gastrointestinal tumors, central nervous system tumors and malignant melanoma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Morphological and quantitative analysis on alpha-tubulin modifications in glioblastoma cells
Gadau SD
Neuroscience Letters, 687, 111-118 (2018)
S Geiger et al.
Oral surgery, oral medicine, and oral pathology, 60(5), 517-523 (1985-11-01)
A 15-year-old boy was referred to the ear, nose, and throat clinic because of a swelling in the upper premolar region. The initial diagnosis of a poorly differentiated soft-tissue sarcoma was made. Further immunohistochemical studies established a definitive diagnosis of...
Podoplanin regulates mammary stem cell function and tumorigenesis by potentiating Wntbeta-catenin signaling
Bresson L, et al.
Development, 145(4), dev160382-dev160382 (2018)
The type III intermediate filament vimentin regulates organelle distribution and modulates autophagy
Biskou O, et al.
Testing, 14(1), e0209665-e0209665 (2019)
Jui M Dave et al.
Microcirculation (New York, N.Y. : 1994), 21(4), 333-344 (2014-01-07)
Angiogenesis is a multistep process that requires intricate changes in cell shape to generate new blood vessels. IF are a large family of proteins that play an important structural and functional role in forming and regulating the cytoskeleton. Vimentin, a...

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