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SAB4200731

Sigma-Aldrich

Anti-Pan-Cadherin antibody, Mouse monoclonal

clone CH-19, purified from hybridoma cell culture

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Synonym(s):
Anti-Pan Cadherin

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

CH-19, monoclonal

form

buffered aqueous solution

mol wt

~135 kDa

species reactivity

guinea pig, canine, frog, bovine, human, cat, monkey, rabbit, mouse, ferret, snake, hamster, chicken, Psammomys (sand rat), rat

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 0.125-0.25 μg/mL using HeLa cell extract
immunofluorescence: 5-10 μg/mL using HeLa cells
immunohistochemistry: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CDH1(999)

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vibrant-m

C7855

Anti-Cdh1 antibody,Mouse monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

CH-19, monoclonal

clone

15B8, monoclonal

clone

GC-4, monoclonal

clone

DCS-266, monoclonal

Gene Information

human ... CDH1(999)

Gene Information

human ... CTNNB1(1499)
mouse ... Ctnnb1(12387)
rat ... Ctnnb1(84353)

Gene Information

human ... CDH2(1000)
mouse ... Cdh2(12558)
rat ... Cdh2(83501)

Gene Information

human ... CDH1(999)

species reactivity

guinea pig, canine, frog, bovine, human, cat, monkey, rabbit, mouse, ferret, snake, hamster, chicken, Psammomys (sand rat), rat

species reactivity

chicken, mouse, human, rat, monkey, hamster, bovine, canine

species reactivity

chicken, monkey, mouse, human, rabbit, rat

species reactivity

human

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

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General description

Anti-Pan-Cadherin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the CH-19 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with the synthetic peptide.
N-cadherin, also known as cadherin-2 (CDH2) or neural cadherin (NCAD) is mapped to human chromosome 18q12.1. N-cadherin has ectodomains comprising of 110 amino acidsand is called the extracellular cadherin (EC) domain. EC domains are critical for the adhesion function of N-cadherin.

Specificity

Monoclonal Anti-Pan-Cadherin recognizes Cadherin from human, mouse, monkey, bovine, chicken, rat, ferret, canine, rabbit, pig, guinea pig, hamster, cat, frog, snake and psammomys origin. Monoclonal Anti Pan-Cadherin is reactive against a highly conserved sequence at the cytoplasmic C-terminal of N-Cadherin protein.

Immunogen

synthetic peptide corresponding to the C-terminus of chicken N-Cadherin with an extra N-terminal lysine residue coupled with KLH.

Application

Anti-Pan-Cadherin antibody, Mouse monoclonal may be used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry

Biochem/physiol Actions

N-cadherin plays a key role in the neurulation, which involves transition of neural plate into neural tube. It is present in the synaptic membrane and dimerises upon synaptic induction and mediates synaptic activity. N-cadherin mediates cell−cell adhesion in retina but favors tumor progression in retinoblastoma. It is upregulated in carcinomas and is critical for cell invasion. Irregular distribution of N-cadherin is implicated in dilated cardiomyopathy. Mutations in the CHD2 gene may probably contribute to obsessive-compulsive disorder (OCD) and Tourette disorder (TD).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Structure and function of the N-cadherin/catenin complex in retinoblastoma
Van A, et al.
Investigative Ophthalmology & Visual Science, 43(3), 595-602 (2002)
Structure and biochemistry of cadherins and catenins
Shapiro L and Weis WI
Cold Spring Harbor Perspectives in Biology, a003053-a003053 (2009)
Structure-function analysis of cell adhesion by neural (N-) cadherin
Tamura K, et al.
Neuron, 20(6), 1153-1163 (1998)
Molecular modification of N-cadherin in response to synaptic activity
Tanaka H, et al.
Neuron, 25(1), 93-107 (2000)
Apoptosis-related factors p53, bcl-2 and the defects of force transmission in dilated cardiomyopathy
Tsipis A , et al.
Pathology Research and Practice, 206(9), 625-630 (2010)

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