Anti-IL6 high affinity antibody, Mouse monoclonal

clone IL6-4G1, purified from hybridoma cell culture

Anti-CTL differentiation factor (CDF), Anti-Hybridoma growth factor, Anti-B-cell stimulatory factor 2 (BSF-2), Anti-Interferon beta-2 (IFN-beta-2), Anti-Interleukin-6

Quality Level

biological source


antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies


IL6-4G1, monoclonal


buffered aqueous solution

species reactivity



~1.0 mg/mL


ELISA: suitable
immunoblotting: 2-4 μg/mL using concentrated supernatant of human osteosarcoma MG-63 cells treated with IL1β
immunoprecipitation (IP): 1-2 μg/test using concentrated supernatant of human osteosarcoma MG-63 cells treated with IL1β



UniProt accession no.

shipped in

dry ice

storage temp.


Gene Information

human ... IL6(3569)

General description

Anti-IL6 high-affinity antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the IL6-4G1 hybridoma, produced by the fusion of mouse NS1 myeloma cells and splenocytes from a BALB/c mouse. Interleukin-6 (IL6) is mapped on the human chromosome at 7p15.3.


Monoclonal Anti-IL6 specifically recognizes human IL6 (KD ~4 x 10-11 M as measured by the Biacore system).


recombinant human IL6


Anti-IL6 high-affinity antibody, mouse monoclonal may be used in:
  • immunoblotting
  • immunoprecipitation
  • enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Interleukin-6 (IL6) is a multifunctional cytokine that plays a wide variety of biological roles in numerous systems including the immune, nervous and endocrine systems. It is considered to perform as a primary regulator of acute and chronic inflammatory diseases. IL6 is an excellent target for clinical treatment against autoimmune disorders such as rheumatoid arthritis, juvenile idiopathic arthritis and Castleman′s disease. It is overexpressed in the pathogenesis of myeloma patients. IL6 might have elevated serum IL6 levels in patients with different kinds of malignant disease. IL6 at higher levels are also clinically implemented as biomarkers for acute coronary syndrome (ACS) , unstable angina, cardiovascular disease, heart attack and stroke.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.


Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Elevated levels of interleukin-6 in unstable angina
Biasucci L M, et al.
Circulation, 94(5), 874-877 (1996)
The role of immunotherapy in multiple myeloma
Kocoglu M and Badros A
Pharmaceutical Analysis, 9(1), 3-3 (2016)
Systemic inflammation in C57BL/6J mice receiving dietary aluminum sulfate; up-regulation of the pro-inflammatory cytokines IL-6 and TNF alpha, C-reactive protein (CRP) and miRNA-146a in blood serum
Pogue A I, et al.
Journal of Alzheimer's Disease & Parkinsonism, 7(6), 874-877 (2017)
L M Biasucci et al.
Circulation, 94(5), 874-877 (1996-09-01)
Elevated plasma levels of C-reactive protein have been found in the majority of patients with unstable angina. The evidence of elevated levels of acute-phase proteins in unstable angina is in line with a growing body of evidence that suggests that...
Inflammatory biomarkers in acute coronary syndromes: part I: introduction and cytokines.
Ehrin J Armstrong et al.
Circulation, 113(6), e72-e75 (2006-02-16)

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