SAB4200739

Sigma-Aldrich

Anti-Protein kinase C antibody, Mouse monoclonal

clone MC5, purified from hybridoma cell culture

Synonym(s):
Anti-PKC
NACRES:
NA.44

Quality Level

biological source

mouse

antibody product type

primary antibodies

clone

MC5, monoclonal

form

buffered aqueous solution

mol wt

antigen ~80 kDa

species reactivity

rat, fish, bovine, rabbit, mouse, human, chicken

concentration

~1.0 mg/mL

application(s)

immunoblotting: 1.25-2.5 μg/mL using human bone marrow neuroblast SH-SY5Y cell extract
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

General description

Anti-Protein kinase C antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the MC5 hybridoma produced by the fusion of X-63 myeloma cells and splenocytes from a BALB/c mouse immunized with purified bovine brain protein kinase C1. Protein kinase C (PKC), is a family of phospholipid- and calcium-activated, diacylglycerol (DAG)-dependent serine/threonine kinases.

Specificity

Monoclonal Anti-Protein kinase C antibody recognizes an epitope located within the amino acid residues 296- 317, at the hinge region, close to or at the trypsin cleavage site of Protein kinase C molecule . The antibody specifically recognizes Protein kinase C from human, rat, bovine, mouse, chicken, rabbit and fish origin.

Application

Anti-Protein kinase C antibody has been used in:
  • immunoblotting
  • immunohistochemistry
  • immunofluorescence
  • immunoprecipitation

Biochem/physiol Actions

Protein kinase C (PKC) play a central role in a variety of cell processes, including: intracellular signal transduction, cell proliferation, apoptosis, differentiation, migration and adhesion, tumor promotion, cardiac hypertrophy, angiogenesis, platelet function, inflammation and oncogenesis. Neuronal PKC modulates several functions such as nerve cell excitability, neuronal plasticity and release of neurotransmitters. PKC activity is regulated by two mechanisms- firstly by phosphorylation which regulates the active site and subcellular localization of the enzyme, secondly by secondary messengers which facilitate association of PKC on membrane. The PKC activity is strongly associated with tumor initiation and progression and have been implemented as a target for treatment of cancer progression and diabetic retinopathy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Purified bovine brain protein kinase C

Storage and Stability

Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Protein kinase C Inhibitors selectively modulate dynamics of cell adhesion molecules and cell death in human colon cancer cells
Dukel M, et al.
Cell adhesion & migration, 13(1), 83-97 (2019)
Protein kinase C: structure, function, and regulation
Newton, Alexandra C
Test, 270(48), 28495-28498 (1995)
Inhibition of protein kinase C enhances angiogenesis induced by platelet-derived growth factor C in hyperglycemic endothelial cells
Moriya J and Ferrara N
Cardiovascular Diabetology, 14(1), 19-19 (2015)
R Garg et al.
Oncogene, 33(45), 5225-5237 (2013-12-18)
Since their discovery in the late 1970s, protein kinase C (PKC) isozymes represent one of the most extensively studied signaling kinases. PKCs signal through multiple pathways and control the expression of genes relevant for cell cycle progression, tumorigenesis and metastatic...
S C Kiley et al.
The Journal of biological chemistry, 266(35), 23761-23768 (1991-12-15)
GH4C1 cells, which express Ca(2+)-dependent alpha- and beta- as well as Ca(2+)-independent gamma-, epsilon- and zeta-protein kinase C (PKC) isozymes, provide a cell culture model for studying isozyme-specific properties and functions. Hormonal activation of PKCs regulates the differentiated functions of...

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