SAB4200741

Sigma-Aldrich

Anti-Human IgE antibody, Mouse monoclonal

clone GE-1, purified from hybridoma cell culture

Synonym(s):
Anti-Immunoglubulin E
NACRES:
NA.46
Pricing and availability is not currently available.

Quality Level

biological source

mouse

antibody product type

primary antibodies

clone

GE-1, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

~1.0 mg/mL

application(s)

ELISA: 0.15-0.3 μg/mL using 1 μg/mL Human IgE myeloma for coating
immunoblotting: suitable
immunoprecipitation (IP): 1-2 μg/test using Human IgE myeloma

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

General description

Anti-Human IgE antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the GE-1 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified human IgE myeloma protein. Immunoglobulin E (IgE) is the least abundant immunoglobulin isotype in circulation. IgE is produced by plasma cells and is highly functional in the lung, skin and mucus membranes. Normal levels of IgE in serum are less than 1 μg/ml.

Immunogen

purified human IgE myeloma protein

Application

Anti-Human IgE antibody, Mouse monoclonal has been used in various immunological techniques, including enzyme linked immunosorbent assay (ELISA), immunoblot, immunoprecipitation and IgE quantification using piezoelectric (Pz) crystal bioassay.

Biochem/physiol Actions

Immunoglobulin E (IgE) plays a critical role in allergic reactions and host protection from parasites. Serum IgE levels are highly elevated during an allergic reaction (such as atopic eczema, extrinsic asthma or hay fever, fungal and parasitic infection, atopic disease or primary immunodeficiency). Detection and measurement of total IgE concentrations is crucial for diagnosis, monitoring and treatment of allergy patients. In addition, the recognition and isolation of allergen-specific IgE can lead to progress in understanding of pathophysiologic mechanisms underlying IgE-associated allergic disorders.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Factors affecting human IgE and IgG responses to allergen-like Schistosoma mansoni antigens: Molecular structure and patterns of in vivo exposure
Fitzsimmons CM, et al.
International Archives of Allergy and Immunology, 142, 40-50 (2019)
Immunoglobulin E in health and disease
Amarasekera M, et al.
Asia Pacific Allergy, 1(1), 12-15 (2011)
T J Suutari et al.
Journal of investigational allergology & clinical immunology, 16(5), 296-302 (2006-10-17)
Allergic reactions to cow's milk are common in small children. One of the main protein allergens found in cow's milk is beta-lactoglobulin (beta-Lg). Reindeer and bovine milk both contain related beta-Lg proteins, but the allergenicity of reindeer beta-Lg has not...
R H Buckley et al.
The Journal of clinical investigation, 55(1), 157-165 (1975-01-01)
Concentrations of IgD and IgE were measured in sera from 165 patients with well-defined immunodeficiency in an effort to find information possibly relevant to the roles of antibodies of these classes in host defense. Values for both immunoglobulins were generally...
F Zellweger et al.
Allergy, 71(12), 1652-1661 (2016-10-07)
IgE-associated allergic diseases belong to the most common inflammatory conditions. Their clinical manifestation ranges from mild symptoms to life-threatening episodes. Often patients experience a reduction in physical and psychologic well-being and suffer from a decreased quality of life due to...
Articles
Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.